School of Chemical and Biomolecular Engineering, The University of Sydney, Sydney, 2006, Australia.
SDx Tethered Membranes Pty Ltd, Roseville, Sydney, 2069, Australia.
J Membr Biol. 2020 Aug;253(4):319-330. doi: 10.1007/s00232-020-00130-5. Epub 2020 Jul 24.
In this study, a lipid bilayer membrane model was used, in which the bilayer is tethered to a solid substrate with molecular tethers. The V-I characteristics of the lipid bilayers were found to be non-linear which suggests the presence of pores that are voltage-dependent. At high applied voltages, the conductance reached a limiting value, presumably indicating a limit on the maximum pore size. A decrease in the spacing between tethers (increasing tether density) caused a decrease in the membrane's conductance at high applied voltage, which is consistent with the maximum pore size being determined by the spacing between the tethers. The inclusion of 10 M% cholesterol within the membrane lipid caused a decrease in the membrane conductance. However, the inclusion of higher levels of cholesterol increased the membrane conductance.
在这项研究中，使用了一种脂质双层膜模型，其中双层通过分子系链固定在固体基底上。发现脂质双层的 V-I 特性是非线性的，这表明存在电压依赖性的孔。在高施加电压下，电导达到一个极限值，可能表明最大孔径存在限制。系链之间的间隔减小（系链密度增加）会导致在高施加电压下膜的电导降低，这与最大孔径由系链之间的间隔决定的结论一致。在膜脂质中包含 10 M%胆固醇会导致膜电导降低。然而，包含更高水平的胆固醇会增加膜电导。
