Gjelstad Astrid, Pedersen-Bjergaard Stig
School of Pharmacy, Blindern, Oslo, Norway.
Bioanalysis. 2011 Apr;3(7):787-97. doi: 10.4155/bio.11.13.
The recent societal requirements to explore more environmentaly friendly solutions in the field of sample preparation have gained increasing focus during recent years. A reduction in the consumption of hazardous organic solvent owing to environmental and cost perspectives, small amounts of sample available and time reduction, have been major incentives for scientists to miniaturize existing sample preparation methods. Some of these challenges were addressed by the introduction of electromembrane extraction (EME), a totally new extraction principle where a potential difference is applied across a thin organic membrane immobilized in the pores in the wall of a porous polypropylene membrane. The potential difference is utilized to extract charged analytes of interest from the sample, across the organic membrane, and into an aqueous acceptor solution present inside the lumen of the hollow fiber. This article focuses on the potential of EME in bioanalysis, including discussions of EME performance.
近年来，社会对在样品制备领域探索更环保解决方案的要求日益受到关注。从环境和成本角度来看，减少有害有机溶剂的消耗、样品量少以及时间缩短，这些都是促使科学家将现有样品制备方法小型化的主要因素。电膜萃取（EME）的引入解决了其中一些挑战，这是一种全新的萃取原理，在固定于多孔聚丙烯膜壁孔中的薄有机膜上施加电位差。利用该电位差将目标带电分析物从样品中萃取出来，穿过有机膜，进入中空纤维内腔中的水相接受溶液。本文重点关注电膜萃取在生物分析中的潜力，包括对其性能的讨论。
