Wang Wen, Wang Jian, Song Haihan, Liu Jie, Song Bao, Cao Xuecheng
Traumatic Orthopedic Department, General Hospital of Jinan Military Command, Jinan, PR China.
Genet Test Mol Biomarkers. 2011 Jul-Aug;15(7-8):503-6. doi: 10.1089/gtmb.2010.0264. Epub 2011 Mar 31.
Cytotoxic T-lymphocyte antigen-4 (CTLA-4) is a potent immunoregulatory molecule that suppresses anti-tumor response by down-regulating T-cell activation. CTLA-4 gene +49G/A polymorphism (rs231775) has been reported to be associated with various cancers.
The objective of this study is to investigate the association between CTLA-4 gene +49G/A polymorphism and the susceptibility to osteosarcoma in the Chinese population.
The CTLA-4 variant +49G/A was detected by polymerase chain reaction-restriction fragment length polymorphism in 205 osteosarcoma cases and 216 age-matched healthy controls. Data were analyzed using the Chi-square test.
Frequencies of the CTLA-4 +49AA genotype and the +49A allele were significantly increased in patients with osteosarcoma compared to healthy controls (OR 2.27, p=0.010, and OR 1.41, p=0.015).
Our data suggest that the +49G/A polymorphism of CTLA-4 gene is associated with increased susceptibility to osteosarcoma.
细胞毒性T淋巴细胞抗原4（CTLA-4）是一种强效免疫调节分子，可通过下调T细胞活化来抑制抗肿瘤反应。据报道，CTLA-4基因+49G/A多态性（rs231775）与多种癌症相关。
本研究旨在探讨CTLA-4基因+49G/A多态性与中国人群骨肉瘤易感性之间的关联。
采用聚合酶链反应-限制性片段长度多态性方法，对205例骨肉瘤患者和216例年龄匹配的健康对照者进行CTLA-4基因+49G/A变异检测。采用卡方检验进行数据分析。
与健康对照相比，骨肉瘤患者中CTLA-4 +49AA基因型和+49A等位基因的频率显著升高（优势比2.27，p=0.010；优势比1.41，p=0.015）。
我们的数据表明，CTLA-4基因的+49G/A多态性与骨肉瘤易感性增加有关。
