Department of Pathology, School of Medicine, Kurume University, Kurume, Japan.
Cancer Sci. 2011 Jul;102(7):1432-6. doi: 10.1111/j.1349-7006.2011.01946.x. Epub 2011 May 9.
Adult T-cell leukemia/lymphoma (ATLL) is a T-cell malignancy associated with HTLV-1. The HTLV-1 provirus genome has the pX region that encodes tax and HTLV-1 basic leucine zipper factor (HBZ). Previous studies have reported that the tax gene is expressed in few ATLL cases, but the HBZ gene in all ATLL cases. In this study, we used HBZ gene in situ hybridization (HBZ-ISH) for detection of the HBZ gene in formalin-fixed paraffin-embedded tissues. This method showed that all cases (n = 19) were positive for the ATLL cell line (MT-1, MT-2, and MT-4) and ATLL mouse model (HBZ-Tg mice and NOD/SCID/β2-microglobulin(null) mice with ATLL transplanted), and the HBZ gene was also detected in all human ATLL cases (n = 16). The percentage of positive cells in HBZ-ISH was 5-70%. Immunohistochemical staining for Tax protein showed positivity in seven of 11 cases in NOD/SCID/β2-microglobulin(null) mice with ATLL transplanted and in six of eight human ATLL cases, but the percentage of positive cells was very low (range, 1-5%). Although HBZ-ISH is unsuitable to detect HTLV-1 clonality, this method is convenient and can be useful for the histological diagnosis of ATLL in HTLV-1 sero-indeterminate patients.
成人 T 细胞白血病/淋巴瘤（ATLL）是一种与 HTLV-1 相关的 T 细胞恶性肿瘤。HTLV-1 前病毒基因组具有编码 tax 和 HTLV-1 碱性亮氨酸拉链因子（HBZ）的 pX 区域。先前的研究报告称，tax 基因在少数 ATLL 病例中表达，但 HBZ 基因在所有 ATLL 病例中表达。在这项研究中，我们使用 HBZ 基因原位杂交（HBZ-ISH）检测福尔马林固定石蜡包埋组织中的 HBZ 基因。该方法显示所有病例（n=19）均为 ATLL 细胞系（MT-1、MT-2 和 MT-4）和 ATLL 小鼠模型（HBZ-Tg 小鼠和 NOD/SCID/β2-微球蛋白（null）小鼠移植 ATLL）阳性，并且在所有人类 ATLL 病例（n=16）中也检测到 HBZ 基因。HBZ-ISH 阳性细胞的百分比为 5-70%。免疫组织化学染色显示 Tax 蛋白在 NOD/SCID/β2-微球蛋白（null）小鼠移植 ATLL 的 11 例中有 7 例和 8 例人类 ATLL 中有 6 例为阳性，但阳性细胞的百分比非常低（范围为 1-5%）。虽然 HBZ-ISH 不适合检测 HTLV-1 克隆性，但这种方法方便，可用于 HTLV-1 血清不确定患者的 ATLL 组织学诊断。
