Marin Oscar, Hasui Kazuhisa, Remondegui Carlos, Sato Eiichi, Aye Moe Moe, Takenouchi Norihiro, Izumo Shuji, Tajima Kazuo
Department of Pathology, Pablo Soria Hospital, Argentina.
Pathol Int. 2002 May-Jun;52(5-6):348-57. doi: 10.1046/j.1440-1827.2002.01356.x.
Human T-cell leukemia virus type 1 (HTLV-1) infection is prevalent in native Americans living in the Andes. Some of their malignant lymphomas (ML) show a peculiar histology suggestive of adult T-cell leukemia/lymphoma (ATLL). To determine whether ML resembling ATLL are indeed ATLL, re-analysis of 34 cases occurring in Jujuy, a province of Argentina, was conducted, concentrating on immunological phenotype, integration of HTLV-1 proviral DNA, expression of HTLV-1 p40Tax and p27Rex, and infection of Epstein-Barr virus (EBV). The ML were 22 cases of mature peripheral T-cell and natural killer (NK)-cell neoplasm (mT/NKN), 11 B-cell malignant neoplasms and one Hodgkin's lymphoma. Polymerase chain reaction against the HTLV-1 proviral DNA, using DNA extracted from paraffin sections, indicated integration of the HTLV-1 proviral DNA in three cases of eight mT/NKN. Two other cases of mT/NKN were positive for anti-HTLV-1 antibodies. Expression of p40Tax and p27Rex was detected in all five of these mT/NKN cases associated with HTLV-1. As such, these five mT/NKN were rediagnosed as ATLL. In situ hybridization signals for EBV-encoded small nuclear early region-1 were detected in nine cases of mT/NKN, of which five cases of NK-cell lymphoma were found to have cytoplasmic CD3 expression, a CD56 phenotype and positivity of TIA1. According to the new World Health Organization classification, the mT/NKN class includes five cases of ATLL and five cases of NK-cell lymphomas. The five cases of ATLL were of native American extraction from an HTLV-1-endemic area around Jujuy, north-west Argentina.
1型人类T细胞白血病病毒（HTLV-1）感染在生活于安第斯山脉的美洲原住民中很普遍。他们的一些恶性淋巴瘤（ML）呈现出一种特殊的组织学特征，提示成人T细胞白血病/淋巴瘤（ATLL）。为了确定类似ATLL的ML是否确实为ATLL，对阿根廷胡胡伊省发生的34例病例进行了重新分析，重点关注免疫表型、HTLV-1前病毒DNA的整合、HTLV-1 p40Tax和p27Rex的表达以及爱泼斯坦-巴尔病毒（EBV）感染情况。这些ML包括22例成熟外周T细胞和自然杀伤（NK）细胞肿瘤（mT/NKN）、11例B细胞恶性肿瘤和1例霍奇金淋巴瘤。使用从石蜡切片中提取的DNA进行针对HTLV-1前病毒DNA的聚合酶链反应，结果显示在8例mT/NKN中有3例存在HTLV-1前病毒DNA的整合。另外2例mT/NKN抗HTLV-1抗体呈阳性。在所有这5例与HTLV-1相关的mT/NKN病例中均检测到p40Tax和p27Rex的表达。因此，这5例mT/NKN被重新诊断为ATLL。在9例mT/NKN中检测到EBV编码的小核早期区域-1的原位杂交信号，其中5例NK细胞淋巴瘤被发现具有细胞质CD3表达、CD56表型和TIA1阳性。根据世界卫生组织的新分类，mT/NKN类别包括5例ATLL和5例NK细胞淋巴瘤。这5例ATLL均来自阿根廷西北部胡胡伊附近HTLV-1流行地区的美洲原住民。
