Tian Zhong, Yin Min, Ma Hongmei, Zhu Longzhang, Shen Hebei, Jia Nengqin
Department of Chemistry, Life and Environmental Science College, Shanghai Normal University, Shanghai 200234, China.
J Nanosci Nanotechnol. 2011 Feb;11(2):953-8. doi: 10.1166/jnn.2011.3100.
The novel supramolecular complexes were prepared with a water-insoluble anticancer drug camptothecin (CPT) loading onto functionalized multiwalled carbon nanotubes via pi-stacking, in order to improve their solubility and antitumor activity. The multiwalled carbon nanotubes were firstly coated with the tri-block copolymer (Pluronic P123) to render high aqueous solubility. The copolymer-coated multiwalled carbon nanotubes can effectively form non-covalent supramolecular complexes with camptothecin. The supramolecular assembly of the complexes (f-MWNTs-CPT) were systematically characterized by transmission electron microscopy (TEM), UV-vis spectrophotometry (UV), fluorescence spectrophotometry, atomic force microscopy (AFM) and electrochemical impedance spectroscopy (EIS). Furthermore, in vitro cytotoxicity studies of f-MWNTs-CPT supramolecular complexes using the MTT assay exhibit enhanced antitumor activity, suggesting that the functionalized multiwalled carbon nanotubes can facilitate intracellular delivery of anticancer drug and improve drug activity.
通过π-堆积作用，将水不溶性抗癌药物喜树碱（CPT）负载到功能化多壁碳纳米管上，制备了新型超分子复合物，以提高其溶解度和抗肿瘤活性。首先用三嵌段共聚物（普朗尼克P123）包覆多壁碳纳米管，使其具有高水溶性。共聚物包覆的多壁碳纳米管能与喜树碱有效形成非共价超分子复合物。通过透射电子显微镜（TEM）、紫外可见分光光度法（UV）、荧光分光光度法、原子力显微镜（AFM）和电化学阻抗谱（EIS）对复合物（f-MWNTs-CPT）的超分子组装进行了系统表征。此外，使用MTT法对f-MWNTs-CPT超分子复合物进行的体外细胞毒性研究显示其抗肿瘤活性增强，这表明功能化多壁碳纳米管可促进抗癌药物的细胞内递送并提高药物活性。
