Therapeutics Research Centre, School of Medicine, Princess Alexandra Hospital, The University of Queensland, Brisbane, Queensland, Australia.
Nanotoxicology. 2012 Mar;6(2):173-85. doi: 10.3109/17435390.2011.569092. Epub 2011 Apr 1.
Systematic studies probing the effects of nanoparticle surface modification and formulation pH are important in nanotoxicology and nanomedicine. In this study, we use laser-scanning fluorescence confocal microscopy to evaluate nanoparticle penetration in viable excised human skin that was intact or tape-stripped. Quantum dot (QD) fluorescent nanoparticles with three surface modifications: Polyethylene glycol (PEG), PEG-amine (PEG-NH₂) and PEG-carboxyl (PEG-COOH) were evaluated for human skin penetration from aqueous solutions at pH 7.0 and at pHs of solutions provided by the QD manufacturer: 8.3 (PEG, PEG-NH₂) and 9.0 (PEG-COOH). There was some penetration into intact viable epidermis of skin for the PEG-QD at pH 8.3, but not at pH 7.0 nor for any other QD at the pHs used. Upon tape stripping 30 strips of stratum corneum, all QDs penetrated through the viable epidermis and into the upper dermis within 24 h.
系统研究探测纳米颗粒表面修饰和配方 pH 值对纳米毒理学和纳米医学非常重要。在这项研究中，我们使用激光扫描荧光共焦显微镜来评估完整或胶带剥离的活人体人皮肤中纳米颗粒的穿透情况。我们评估了三种表面修饰的量子点（QD）荧光纳米颗粒在 pH 值为 7.0 和 QD 制造商提供的溶液 pH 值（PEG、PEG-NH₂ 为 8.3，PEG-COOH 为 9.0）时从水溶液中对人体皮肤的穿透情况。PEG-QD 在 pH 值为 8.3 时有一些穿透到完整的活表皮，但在 pH 值为 7.0 时以及在使用的任何其他 pH 值时均没有穿透。在胶带剥离 30 条角质层后，所有 QD 在 24 小时内穿透到活表皮并进入上真皮。
