Department of Pharmacy, Faculty of Pharmacy, Al-Zaytoonah University of Jordan, Amman, 11733, Jordan.
Department of Pharmaceutics & Pharmaceutical Technology, School of Pharmacy, The University of Jordan, Amman, 11942, Jordan.
Sci Rep. 2019 Apr 8;9(1):5796. doi: 10.1038/s41598-019-42047-7.
Herein, a library of gold nanorods (GNR) decorated with polyethylene glycol-thiol (PEG-SH) containing different functionalities were synthesized and characterized by optical absorption spectroscopy, zeta potential, dynamic light scattering (DLS), transmission electron microscope (TEM) and proton nuclear magnetic resonance (H-NMR). The colloidal stability of GNR when exposed to skin, and their preferential accumulation into excised human skin layers were investigated. Confocal laser scanning microscopy, transmission electron microscope (TEM) and inductively coupled plasma-optical emission spectroscopy (ICP-OES) were utilized to track the penetration of GNR into different skin layers. The results demonstrated that cholesterol-PEG coated GNR were preferentially loaded up in the upper layers of skin (stratum corneum), while phospholipid-PEG coated counterparts were drastically deposited in skin dermis. Neutral methoxy-PEG-coated GNR were distributed in both SC and dermis skin layers, while charged GNR (anionic-carboxylic acid-PEG-GNR and cationic-amine-PEG-GNR) revealed a minimal accumulation into skin. DSPE-PEG-GNR and Chol-PEG-GNR demonstrated antibacterial activities against Staphylococcus aureus (S aureus) at MIC values of 0.011 nM and 0.75 nM, respectively. Photothermal treatment for S. aureus at sub-MIC concentrations resulted in a significant bactericidal effect when using Chol-PEG-GNR but not DSPE-PEG-GNR. Gold-based nanoscale systems have great value as a promising platform for skin diseases therapy.
本文合成了一系列金纳米棒（GNR），并通过光学吸收光谱、Zeta 电位、动态光散射（DLS）、透射电子显微镜（TEM）和质子核磁共振（H-NMR）对其进行了表征。研究了 GNR 在暴露于皮肤时的胶体稳定性及其对离体人皮肤层的优先积累。利用共聚焦激光扫描显微镜、透射电子显微镜（TEM）和电感耦合等离子体-光学发射光谱（ICP-OES）跟踪 GNR 进入不同皮肤层的穿透情况。结果表明，胆固醇-PEG 包裹的 GNR 优先加载在皮肤的上层（角质层），而磷脂-PEG 包裹的 GNR 则大量沉积在皮肤真皮层。中性甲氧基-PEG 包裹的 GNR 分布在 SC 和真皮皮肤层中，而带电荷的 GNR（阴离子-羧酸-PEG-GNR 和阳离子-胺-PEG-GNR）在皮肤中的积累则很少。DSPE-PEG-GNR 和 Chol-PEG-GNR 的 MIC 值分别为 0.011 nM 和 0.75 nM，对金黄色葡萄球菌（S aureus）具有抗菌活性。在亚 MIC 浓度下对 S. aureus 进行光热治疗时，使用 Chol-PEG-GNR 而非 DSPE-PEG-GNR 可显著杀菌。基于金的纳米级系统作为治疗皮肤病的有前途的平台具有很高的价值。
