Carvalho Carolina Montagn, Lacerda Juliana A, dos Santos Neto Fernando P, de Castro Isabelle Cardoso Vieira, Ramos Thaís Andrade, de Lima Flávia Oliveira, de Cerqueira Luz João Gualberto, Ramalho Maria José P, dos Santos Jean N, Pinheiro Antonio L B
Center of Biophotonics, School of Dentistry, Federal University of Bahia, Salvador, BA, Brazil.
Photomed Laser Surg. 2011 Apr;29(4):245-54. doi: 10.1089/pho.2009.2685.
The aim of this study was to evaluate, by light microscopy, the effects of laser phototherapy (LPT) at 780 nm or a combination of 660 and 790 nm, on the inflammatory process of the rat temporomandibular joint (TMJ) induced by carrageen.
Temporomandibular disorders (TMDs) are frequent in the population and generally present an inflammatory component. Previous studies have evidenced positive effects of laser phototherapy on TMDs. However, its mechanism of action on the inflammation of the TMJ is not known yet.
Eighty-five Wistar rats were divided into 9 groups: G1, Saline; G2, Saline + LPT IR; G3, Saline + LPT IR + R; G4, Carrageenan; G5, Carrageenan + LPT IR; G6, Carrageenan + LPT IR + R; G7, previous LPT + Carrageenan; G8, previous LPT + carrageenan + LPT IR; and G9, previous LPT + carrageenan + LPT IR + R, and then subdivided in subgroups of 3 and 7 days. After animal death, specimens were taken, routinely cut and stained with HE, Sirius Red, and Toluidine Blue. Descriptive analysis of components of the TMJ was done. The synovial cell layers were counted.
Injection of saline did not produced inflammatory reaction and the irradiated groups did not present differences compared to nonirradiated ones. After carrageenan injection, intense inflammatory infiltration and synovial cell layers proliferation were observed. The infrared irradiated group presented less inflammation and less synovial cell layers number compared to other groups. Previous laser irradiation did not improve the results.
It was concluded that the LPT presented positive effects on inflammatory infiltration reduction and accelerated the inflammation process, mainly with IR laser irradiation. The number of synovial cell layers was reduced on irradiated group.
本研究旨在通过光学显微镜评估780nm激光光疗（LPT）或660nm与790nm联合激光光疗对角叉菜胶诱导的大鼠颞下颌关节（TMJ）炎症过程的影响。
颞下颌关节紊乱病（TMDs）在人群中很常见，通常存在炎症成分。先前的研究已证明激光光疗对TMDs有积极作用。然而，其对TMJ炎症的作用机制尚不清楚。
85只Wistar大鼠分为9组：G1，生理盐水组；G2，生理盐水+红外LPT组；G3，生理盐水+红外LPT+R组；G4，角叉菜胶组；G5，角叉菜胶+红外LPT组；G6，角叉菜胶+红外LPT+R组；G7，预先LPT+角叉菜胶组；G8，预先LPT+角叉菜胶+红外LPT组；G9，预先LPT+角叉菜胶+红外LPT+R组，然后再分为3天和7天的亚组。动物死亡后，取材，常规切片，用苏木精-伊红（HE）、天狼星红和甲苯胺蓝染色。对角叉菜胶诱导的大鼠颞下颌关节组织学变化进行描述性分析，计数滑膜细胞层数。
注射生理盐水未产生炎症反应，与未照射组相比，照射组无差异。注射角叉菜胶后，观察到强烈的炎症浸润和滑膜细胞层增殖。与其他组相比，红外照射组炎症较轻，滑膜细胞层数较少。预先激光照射并未改善结果。
得出结论，LPT对角叉菜胶诱导的大鼠颞下颌关节炎症浸润减少有积极作用，主要是红外激光照射加速了炎症进程。照射组滑膜细胞层数减少。
