TaSRG，一种小麦转录因子，显著影响转基因水稻和拟南芥的耐盐性。
TaSRG, a wheat transcription factor, significantly affects salt tolerance in transgenic rice and Arabidopsis.
机构信息
College of Life Science of Hebei Normal University, No. 113 Yuhua East Road, Shijiazhuang, Hebei, China.
出版信息
FEBS Lett. 2011 Apr 20;585(8):1231-7. doi: 10.1016/j.febslet.2011.03.055. Epub 2011 Mar 30.
Based on our microarray-analysis results of the salt tolerant wheat mutant, RH8706-49, an unknown salt-induced gene containing a conserved DUF662 domain was identified and cloned; we named this gene TaSRG (Triticum aestivum Salt Response Gene). Real-time quantitative PCR analyses showed that the expression of this gene was affected by salt, drought, cold, ABA and other stress conditions. The overexpression of TaSRG in wild-type Arabidopsis resulted in increased salt tolerance compared with wild-type plants. In addition, subcellular localisation revealed that TaSRG encodes a protein that was found mainly in the nucleus. Our results suggest that wheat TaSRG might encode a transcription factor that could be utilised for enhancing stress tolerance in a wide range of plants.
基于我们对耐盐小麦突变体 RH8706-49 的基因芯片分析结果，鉴定并克隆了一个含有保守 DUF662 结构域的未知盐诱导基因；我们将这个基因命名为 TaSRG（小麦盐反应基因）。实时定量 PCR 分析表明，该基因的表达受盐、干旱、寒冷、ABA 和其他胁迫条件的影响。在野生型拟南芥中过表达 TaSRG 可提高其耐盐性，与野生型植株相比。此外，亚细胞定位表明 TaSRG 编码的蛋白主要存在于细胞核中。我们的研究结果表明，小麦 TaSRG 可能编码一种转录因子，可用于提高多种植物的胁迫耐受性。
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