Elias Júlio Cesar F, Gonçalves-Vidigal Maria Celeste, Ariani Andrea, Valentini Giseli, Martiniano-Souza Maria da Conceição, Vaz Bisneta Mariana, Gepts Paul
Departamento de Agronomia, Universidade Estadual de Maringá-UEM, Av. Colombo 5790, Maringá 87020-900, Brazil.
BASF BBCC-Innovation Center, 9052 Gent, Belgium.
Plants (Basel). 2021 Jul 30;10(8):1572. doi: 10.3390/plants10081572.
Abiotic stress is a limiting factor for common bean () production globally. The study of the genotypic, phenotypic, and bio-climatic variables in a broad set of accessions may assist the identification of genomic regions involved in the climatic adaptation of the common bean. We conducted a genotyping-by-sequencing analysis using 28,823 SNPs on 110 georeferenced common bean accessions from Brazil to discover associations between SNPs and bio-climatic indexes. The population structure analysis clustered the accessions into two groups corresponding to the Andean and Mesoamerican gene pools. Of the 19 bioclimatic variables, 17 exhibited a significant association with SNPs on chromosomes Pv01, Pv02, Pv03, Pv04, Pv06, Pv09, Pv10, and Pv11 of common bean. Ten candidate genes were associated with specific bio-climatic variables related to temperature and precipitation. The candidate genes associated with this significant Pv09 region encode a Platz transcription factor family protein previously reported to be an essential regulator of drought stress. The SNP markers and candidate genes associated with the bio-climatic variables should be validated in segregating populations for water stress, which could further be used for marker-assisted selection. As a result, bean breeding programs may be able to provide advances in obtaining drought-tolerant cultivars.
非生物胁迫是全球普通菜豆（）生产的限制因素。对大量种质资源的基因型、表型和生物气候变量进行研究，可能有助于识别普通菜豆气候适应性相关的基因组区域。我们对来自巴西的110份地理定位的普通菜豆种质进行了基因分型测序分析，使用了28,823个单核苷酸多态性（SNP）来发现SNP与生物气候指标之间的关联。群体结构分析将这些种质聚类为两组，分别对应安第斯和中美洲基因库。在19个生物气候变量中，有17个与普通菜豆染色体Pv01、Pv02、Pv03、Pv04、Pv06、Pv09、Pv10和Pv11上的SNP表现出显著关联。10个候选基因与与温度和降水相关的特定生物气候变量有关。与这个显著的Pv09区域相关的候选基因编码一种Platz转录因子家族蛋白，此前报道该蛋白是干旱胁迫的重要调节因子。与生物气候变量相关的SNP标记和候选基因应在水分胁迫分离群体中进行验证，这可进一步用于标记辅助选择。因此，菜豆育种计划或许能够在培育耐旱品种方面取得进展。
