The Key Sericultural Laboratory of Agricultural Ministry, College of Biotechnology, Southwest University, Tiansheng Road 1, Beibei, Chongqing 400715, China.
Insect Biochem Mol Biol. 2011 Aug;41(8):572-81. doi: 10.1016/j.ibmb.2011.03.009. Epub 2011 Mar 30.
The patterning of embryos in early stages is a critical process for embryo development. In order to understand the molecular mechanism of early embryogenesis in silkworm, 2-DE combined with MALDI-TOF-MS technologies were used to analyze the proteins from diapause-destined eggs at the germ-band formation stage. From over 1000 spots, 93 were selected for analysis and data were obtained from 59 revealing 42 proteins. Gene Ontology annotation showed these proteins were involved in several biological processes at the germ-band formation stage, including cell stress response and protein folding, cell growth and migration, termination of diapause, and nutrition storage. Prominent among them was a new 80 kDa protein, named Bombyx mori egg protein 80 (BmEP80). BmEP80 was a component of the eggshell which was secreted by follicle cells during the late vitellogenesis stage to early choriogenesis stage (FCs -5 to +10). It disappears during early embryogenesis and RNAi against it resulted in the collapse of eggs, thus it is likely that BmEP80 is a new component of the silkworm vitelline membrane.
胚胎在早期阶段的模式化是胚胎发育的关键过程。为了了解家蚕早期胚胎发生的分子机制，我们使用 2-DE 结合 MALDI-TOF-MS 技术来分析处于原肠胚形成阶段的滞育卵中的蛋白质。在超过 1000 个斑点中，选择了 93 个进行分析，其中 59 个显示出 42 种蛋白质。基因本体论注释表明，这些蛋白质参与了原肠胚形成阶段的几个生物学过程，包括细胞应激反应和蛋白质折叠、细胞生长和迁移、滞育终止以及营养储存。其中一个突出的是一种新的 80 kDa 蛋白，命名为家蚕卵蛋白 80（BmEP80）。BmEP80 是卵壳的一个组成部分，在卵黄发生晚期到早中胚层发生期（FCs-5 到+10）期间由滤泡细胞分泌。它在早期胚胎发生过程中消失，针对它的 RNAi 导致卵的崩溃，因此 BmEP80 可能是家蚕卵黄膜的一个新成分。
