Department of Biochemistry, Basic Medical Sciences Block, Panjab University, Chandigarh, India.
Cell Immunol. 2011;269(1):16-21. doi: 10.1016/j.cellimm.2011.03.004. Epub 2011 Mar 12.
Activated cytotoxic T lymphocyte (CTL) mediated target cell death has been implicated in the development of systemic autoimmune disease like SLE. However, the role of soluble granzyme B and its relationship with CTL activity and disease activity is still unknown. In this study, we evaluated role of soluble granzyme B and cytotoxic T lymphocyte activity in SLE patients. The soluble granzyme B was measured in the serum by an enzyme-linked immunosorbent assay while cytotoxic T lymphocyte activity was measured by flow cytometry. The disease activity was determined by using SLE Disease Activity Index (SLEDAI) score. Cytotoxic T lymphocyte activity was increased and strongly associated with disease activity. The soluble granzyme B levels were higher in SLE patients and associated with various clinical features like reduced complement components; C3 & C4 and skin lesion. The soluble granzyme B levels were also sturdily related with severity of the disease. The findings of this study suggest that excessive secretion of soluble granzyme B and enhanced activity of cytotoxic T lymphocyte may play a vital role in the pathogenesis of SLE and organ damage. Also, evaluation of soluble granzyme B may be helpful in monitoring the clinical features associated with activated CTL in SLE.
已发现细胞毒性 T 淋巴细胞（CTL）介导的靶细胞死亡与全身性自身免疫性疾病（如 SLE）的发生有关。然而，可溶性颗粒酶 B 的作用及其与 CTL 活性和疾病活动的关系仍不清楚。在这项研究中，我们评估了可溶性颗粒酶 B 和 CTL 活性在 SLE 患者中的作用。通过酶联免疫吸附试验测定血清中的可溶性颗粒酶 B，通过流式细胞术测定 CTL 活性。通过 SLE 疾病活动指数（SLEDAI）评分确定疾病活动度。CTL 活性增加，并与疾病活动密切相关。SLE 患者的可溶性颗粒酶 B 水平升高，并与各种临床特征相关，如补体成分减少；C3 和 C4 以及皮肤损伤。可溶性颗粒酶 B 水平也与疾病的严重程度密切相关。这项研究的结果表明，可溶性颗粒酶 B 的过度分泌和 CTL 活性的增强可能在 SLE 的发病机制和器官损伤中发挥重要作用。此外，可溶性颗粒酶 B 的评估可能有助于监测与 SLE 中活化 CTL 相关的临床特征。
