应用环介导等温扩增法鉴定 PCR 核糖型 027 艰难梭菌。
Use of the loop-mediated isothermal amplification method for identification of PCR ribotype 027 Clostridium difficile.
机构信息
Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan.
出版信息
J Med Microbiol. 2011 Aug;60(Pt 8):1126-1130. doi: 10.1099/jmm.0.029587-0. Epub 2011 Apr 1.
The loop-mediated isothermal amplification (LAMP) assay detecting the slpA gene of slpA sequence type gc8 (slpA-gc8) was established for the identification of a hypervirulent Clostridium difficile strain, PCR ribotype 027. Of 107 isolates examined, 27 belonging to PCR ribotype 027 were all positive for the LAMP assay. The remaining 80 isolates were typed into 47 different PCR ribotypes other than type 027, and were negative for the LAMP assay with the exception of two isolates. The sensitivity and specificity of the LAMP method for identification of PCR ribotype 027 were 100 % and 98 %, respectively. The LAMP assay detecting slpA-gc8 is a reliable tool for the identification of PCR ribotype 027 C. difficile. This simple and rapid method will contribute to detection of the hypervirulent strain.
建立了一种检测 slpA-gc8 的环介导等温扩增（LAMP）检测 slpA 基因的方法，用于鉴定一种毒力超强的艰难梭菌菌株，PCR 核糖体分型 027。在检测的 107 株分离株中，27 株属于 PCR 核糖体分型 027 的分离株均为 LAMP 检测阳性。其余 80 株被分为除 027 型以外的 47 种不同的 PCR 核糖体分型，除两株外，LAMP 检测均为阴性。LAMP 法鉴定 PCR 核糖体分型 027 的灵敏度和特异性分别为 100%和 98%。检测 slpA-gc8 的 LAMP 检测法是鉴定 PCR 核糖体分型 027 艰难梭菌的可靠工具。这种简单快速的方法将有助于检测毒力超强的菌株。
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