Departamento de Biotecnología de Alimentos, Instituto de la Grasa, Consejo Superior de Investigaciones Científicas (CSIC), Avda. Padre García Tejero, 4; 41012 Sevilla, Spain.
Instituto de Recursos Naturales y Agrobiología, Consejo Superior de Investigaciones Científicas (CSIC), P.O. Box 1052, 41080 Sevilla, Spain.
Int J Syst Evol Microbiol. 2014 Sep;64(Pt 9):2980-2985. doi: 10.1099/ijs.0.063032-0. Epub 2014 Jun 9.
Five strains of Gram-stain-positive bacteria were isolated from anomalous fermentations occurring in post-packaging of sealed airtight food-grade plastic pouches of Spanish-style green olives. These isolates could be grouped into two sets, which showed a similarity in their respective 16S rRNA gene sequences of 98.40 and 98.44 % with Propionibacterium acidipropionici NCFB 563 and 98.33 and 98.11 % with Propionibacterium microaerophilum M5(T), respectively, and a similarity of 99.41 % between them. The 16S rRNA gene phylogeny revealed that the isolates grouped into two statistically well-supported clusters separate from P. acidipropionici NCFB 563 and P. microaerophilum M5(T). Enzymic activity profiles as well as fermentation patterns differentiated these two novel bacteria from other members of the genus Propionibacterium. Finally, phenotypic, genotypic and phylogenetic data, supported the proposal of two novel species of the genus Propionibacterium, for which the names Propionibacterium olivae sp. nov. (type strain, IGBL1(T) = CECT 8061(T) = DSM 25436(T)) and Propionibacterium damnosum sp. nov. (type strain, IGBL13(T) = CECT 8062(T) = DSM 25450(T)) are proposed.
从密封的食品级塑料小袋西班牙绿橄榄的包装后异常发酵中分离到五株革兰氏阳性菌。这些分离株可分为两组，其 16S rRNA 基因序列的相似性分别为 98.40%和 98.44%与丙酸杆菌 NCFB 563 和 98.33%和 98.11%与丙酸杆菌 M5(T)，彼此之间的相似性为 99.41%。16S rRNA 基因系统发育分析表明，这些分离株与丙酸杆菌 NCFB 563 和丙酸杆菌 M5(T)分为两个统计学上支持的聚类。酶活性谱和发酵模式将这两种新型细菌与丙酸杆菌属的其他成员区分开来。最后，表型、基因型和系统发育数据支持了丙酸杆菌属两个新种的提议，分别命名为丙酸杆菌橄榄亚种（模式株，IGBL1(T)=CECT 8061(T)=DSM 25436(T)）和丙酸杆菌 damnosum 亚种（模式株，IGBL13(T)=CECT 8062(T)=DSM 25450(T)）。
