Minkowski M, Lebel B, Arnould A, Dy M
CNRS URA 122, INSERM U 25, Necker Hospital, Paris, France.
Exp Hematol. 1990 Dec;18(11):1158-63.
Recombinant human interleukin 3 (rhIL-3) induces an increase in histamine production by human bone marrow, fetal liver, and cord blood cells. This phenomenon, already significant after 3 days of incubation, is strikingly enhanced following either enrichment in immature cell subpopulations or CD8+ cell depletion. It results from an increase in histamine synthesis because of 1) the low level of histamine cell content before any incubation, 2) the parallel increase in both extra- and intracellular histamine levels in response to rhIL-3, and 3) the early IL-3-induced increase in L-histidine decarboxylase (HDC; EC 4.1.1.22) activity. Moreover, rhIL-3 has no similar activity on adult peripheral blood cells, suggesting that it might be specific to the hemopoietic system.
重组人白细胞介素3（rhIL-3）可诱导人骨髓、胎肝和脐血细胞产生的组胺增加。这种现象在培养3天后就已很明显，在未成熟细胞亚群富集或CD8 +细胞耗竭后会显著增强。这是由于组胺合成增加所致，原因如下：1）在任何培养之前组胺细胞含量水平较低；2）响应rhIL-3时细胞外和细胞内组胺水平同时增加；3）IL-3早期诱导L-组氨酸脱羧酶（HDC；EC 4.1.1.22）活性增加。此外，rhIL-3对成人外周血细胞没有类似活性，这表明它可能对造血系统具有特异性。
