Lebel B, Schneider E, Piquet-Pellorce C, Machavoine F, Kindler V, Luffau G, Dy M
INSERM U 25 Hôpital Necker, Paris, France.
J Immunol. 1990 Aug 15;145(4):1222-6.
Antigenic challenge of Nippostrongylus brasiliensis-infected mice induces a striking increase in histidine decarboxylase (HDC) activity in both spleen and bone marrow cells. This enhancement takes place within 1 h after injection, with a maximum at 4 h and a return to pretreatment values 20 h later. It is associated with the appearance of IL-3 in the sera of these mice. In addition, the intracellular histamine content in both hematopoietic organs is concomitantly increased. A similar injection of worm Ag into normal mice has no significant effect. Comparable enhancement of HDC activity and intracellular histamine content with almost identical kinetics is promoted by i.v. injection of rIL-3 into normal mice. Moreover, HDC levels in infected mice are increased to the same extent in response to either specific antigen or rIL-3 injection. Taken together these results support the conclusion that antigenic challenge of immunized mice induces endogeneous IL-3 which, in turn, promotes a rapid increase in histamine synthesis in hematopoietic organs.
用巴西日圆线虫感染小鼠后进行抗原攻击，可使脾脏和骨髓细胞中的组氨酸脱羧酶（HDC）活性显著增加。这种增强在注射后1小时内发生，4小时达到最大值，20小时后恢复到预处理值。这与这些小鼠血清中IL-3的出现有关。此外，两个造血器官中的细胞内组胺含量也随之增加。向正常小鼠注射类似的蠕虫抗原则没有显著影响。通过向正常小鼠静脉注射rIL-3，可促进HDC活性和细胞内组胺含量以几乎相同的动力学进行类似的增强。此外，感染小鼠的HDC水平在注射特异性抗原或rIL-3后会增加到相同程度。综上所述，这些结果支持以下结论：免疫小鼠的抗原攻击诱导内源性IL-3，进而促进造血器官中组胺合成的快速增加。
