Khire J, Das M
Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia 19104-6059.
FEBS Lett. 1990 Oct 15;272(1-2):69-72. doi: 10.1016/0014-5793(90)80451-n.
Human A431 cells express a 100 kDa EGF-receptor homolog that contains only the external domain. The kinetics of its maturation and export are slow and comparable to those of the transmembrane receptor. Here we demonstrate that exogenously added EGF stimulates post-translational processing and export of this receptor through a pathway that is insensitive to inhibitors of protein synthesis. The results suggest that EGF may influence one or more of the rate determining steps that control receptor export from endoplasmic reticulum. This may constitute one of the pathways by which EGF regulates the expression of its receptor.
人A431细胞表达一种仅含胞外结构域的100 kDa表皮生长因子（EGF）受体同源物。其成熟和输出的动力学过程缓慢，与跨膜受体的动力学过程相当。在此我们证明，外源添加的EGF通过一条对蛋白质合成抑制剂不敏感的途径刺激该受体的翻译后加工和输出。结果表明，EGF可能影响控制受体从内质网输出的一个或多个速率决定步骤。这可能构成EGF调节其受体表达的途径之一。
