Department of Food and Environmental Sciences, University of Helsinki, P.O. Box 56, 00014 Helsinki, Finland.
J Ind Microbiol Biotechnol. 2011 Oct;38(10):1719-27. doi: 10.1007/s10295-011-0961-4. Epub 2011 Apr 2.
The antifouling potential of electric polarization combined and not combined with biocides was studied in nonsaline warm water with high organic content. Deinococcus geothermalis is a bacterium known for forming colored biofilms in paper machines and for its persistence against cleaning and chemical treatments. When D. geothermalis biofilms grown for 24 h in simulated paper machine water were exposed to cathodic or cathodically weighted pulsed polarization at least 60% (P < 0.05) of the biofilms were removed from stainless steel (AISI 316L). Biofilm removal by 25 ppm (effective substances 5-25 ppm) of oxidizing biocides (bromochloro-5,5-dimethylhydantoin, 2,2-dibromo-2-cyanoacetamide, peracetic acid) increased to 70% when combined with cathodically weighted pulsed polarization. Using a novel instrument that allows real-time detection of reactive oxygen species (ROS) we showed that the polarization program effective in antifouling generated ROS in a pulsed manner on the steel surface. We thus suggest that the observed added value of oxidative biocides combined with polarization depended on ROS. This suggestion was supported by the finding that a reductive biocide, methylene bisthiocyanate, counteracted the antifouling effect of polarization.
在高有机物含量的非盐水暖水中研究了电极化与杀生剂联合和不联合的防污潜力。地杆菌属是一种已知在造纸机中形成有色生物膜的细菌，并且对清洁和化学处理具有很强的抵抗力。当在模拟造纸机水中生长 24 小时的地杆菌属生物膜暴露于阴极或阴极加权脉冲极化时，不锈钢（AISI 316L）上至少有 60%（P<0.05）的生物膜被去除。当与阴极加权脉冲极化结合使用时，氧化杀生剂（溴氯-5,5-二甲基海因、2,2-二溴-2-氰基乙酰胺、过氧乙酸）的 25 ppm（有效物质 5-25 ppm）可将生物膜去除率提高到 70%。使用一种新型仪器可以实时检测活性氧（ROS），我们发现，在钢表面上以脉冲方式产生 ROS 的极化程序在防污方面是有效的。因此，我们认为与极化结合使用的氧化杀生剂的附加值取决于 ROS。这一假设得到了还原性杀生剂亚甲基双硫氰酸酯抵消极化防污效果的发现的支持。
