Pérez-Roa Rodolfo E, Anderson Marc A, Rittschof Dan, Hunt Christopher G, Noguera Daniel R
Department of Civil and Environmental Engineering, University of Wisconsin-Madison, Madison, WI, USA.
Biofouling. 2009;25(6):563-71. doi: 10.1080/08927010902995564.
The role of reactive oxygen species (ROS) in electrochemical biofouling inhibition was investigated using a series of abiotic tests and settlement experiments with larvae of the barnacle Amphibalanus amphitrite, a cosmopolitan fouler. Larval settlement, a measure of biofouling potential, was reduced from 43% +/- 14% to 5% +/- 6% upon the application of pulsed electric signals. The application of ROS scavengers such as glutathione and catalase counteracted the inhibitory effects of the electric signals, allowing settlement, and thus indicating that ROS are antifouling agents. Based on the experimental evidence, the proposed mechanism for ROS-based fouling prevention with interdigitated electrodes involved the electrochemical generation of hydrogen peroxide by oxygen reduction, and its likely reduction to hydroxyl radicals. Either hydroxyl radicals or products of hydroxyl radical reactions appeared to be the main deterrents of larval settlement.
利用一系列非生物测试以及与全球分布的污损生物藤壶（Amphibalanus amphitrite）幼虫进行的附着实验，研究了活性氧（ROS）在电化学生物污损抑制中的作用。幼虫附着是生物污损潜力的一种衡量指标，施加脉冲电信号后，其从43%±14%降至5%±6%。施用谷胱甘肽和过氧化氢酶等ROS清除剂可抵消电信号的抑制作用，使幼虫能够附着，从而表明ROS是防污剂。基于实验证据，所提出的利用叉指电极基于ROS防污的机制涉及通过氧还原电化学生成过氧化氢，以及其可能还原为羟基自由基。羟基自由基或羟基自由基反应产物似乎是幼虫附着的主要阻碍因素。
