Department of Hematology, Postgraduate Institute of Medical Education and Research, India.
Pediatr Blood Cancer. 2011 Jul 15;57(1):18-29. doi: 10.1002/pbc.22954. Epub 2011 Apr 1.
Applications of flow cytometry in pediatric cancers have expanded substantially in recent years. In acute leukemias, the commonest childhood cancer, flow cytometry can now define complex antigenic profiles that are associated with specific cytogenetic/molecular defects and can also directly identify BCR-ABL fusion protein. Flow cytometry based scoring system has been described for diagnosis of myelodysplastic syndromes. In solid tumors, flow cytometry was previously used mainly to determine DNA content for prognosis; however, recent studies in children with neuroblastoma and Ewing sarcoma have identified its diagnostic utility. In this review, we will discuss the current and future applications of flow cytometry in pediatric hematology-oncology.
近年来，流式细胞术在儿科癌症中的应用有了显著的扩展。在最常见的儿童癌症——急性白血病中，流式细胞术现在可以确定与特定细胞遗传学/分子缺陷相关的复杂抗原谱，并且还可以直接识别 BCR-ABL 融合蛋白。基于流式细胞术的评分系统已被描述用于诊断骨髓增生异常综合征。在实体瘤中，流式细胞术以前主要用于确定 DNA 含量以进行预后判断；然而，最近在患有神经母细胞瘤和尤文肉瘤的儿童中的研究已经确定了其诊断效用。在这篇综述中，我们将讨论流式细胞术在儿科血液肿瘤学中的当前和未来应用。
