Ota K, Takamoto S
Gan To Kagaku Ryoho. 1982 Jul;9(7):1140-50.
The application of flow cytometry (FCM) in the field of clinical oncology is reviewed. Since the development of the instruments, it has already passed more than a decade and, these days, FCM is utilized in a variety of fields, both basic and clinical researches. FCM has been getting familiar also in Japan for these several years and now more than fifty instruments are running in many laboratories. In this issue, the application of FCM to clinical oncology is described by several authors from different fields. In this review, the definition of FCM, the principle how it works, its advantages and the history of its development are briefly introduced. Furthermore, the application of FCM by measurements of cellular DNA content for estimation of cell cycle distribution and ploidy studies or by measurements of cell surface fluorescence for detection of cell surface antigens is reviewed in hematology, immunology, cell kinetics of cancer and chemotherapy, automated cytology, chromosome analysis and other fields.
本文综述了流式细胞术（FCM）在临床肿瘤学领域的应用。自该仪器问世以来，已经过去了十多年，如今，FCM已被应用于基础研究和临床研究等多个领域。近年来，FCM在日本也逐渐为人所熟知，目前有五十多台仪器在许多实验室中运行。在本期中，来自不同领域的几位作者描述了FCM在临床肿瘤学中的应用。在这篇综述中，简要介绍了FCM的定义、工作原理、优点及其发展历程。此外，还综述了FCM在血液学、免疫学、癌症细胞动力学与化疗、自动化细胞学、染色体分析及其他领域的应用，这些应用包括通过测量细胞DNA含量来估计细胞周期分布和进行倍性研究，或通过测量细胞表面荧光来检测细胞表面抗原。
