Kowal-Vern A, Gonzalez-Crussi F, Turner J, Trujillo Y P, Chou P, Herman C, Castelli M, Walloch J
Department of Pathology, Loyola University Medical Center, Maywood, Illinois 60153.
Cancer Res. 1990 Sep 15;50(18):6023-7.
Rhabdomyosarcoma is the most common malignant soft-tissue tumor in childhood, with an overall 3-year disease-free survival of 73%. DNA content is known to correlate with prognosis and therapy response in many cancers. To determine the role of DNA content in rhabdomyosarcoma, 23 tumor samples were studied retrospectively: 18 primary tumors and 5 post-chemotherapy recurrences or specimens obtained at second-look surgeries. The DNA analysis was performed on disaggregated paraffin-embedded tissue nuclei by flow and image cytometry and correlated with the histology and clinical history. Of the primary tumors 4 were diploid, 4 polyploid, and 10 aneuploid (9 with a single aneuploid G0G1 peak and 1 multiploid) by flow cytometry. The concordance rate between flow and image cytometry was 19 of 23 (83%); one case did not have flow cytometry available. Most embryonal rhabdomyosarcomas were aneuploid (10 of 12; 83%), and they had a high incidence of recurrence in Stages III and IV (4 of 12; 33%). Although aneuploidy in pediatric cancers may predict a therapeutic response and good prognosis, this was not supported by our findings in rhabdomyosarcoma. The tumor DNA content correlated with the clinical stage but not with the patient's clinical course or tumor histopathological type. DNA content did not appear to be as important a prognostic tool as tumor stage.
横纹肌肉瘤是儿童期最常见的恶性软组织肿瘤，总体3年无病生存率为73%。已知DNA含量与许多癌症的预后及治疗反应相关。为确定DNA含量在横纹肌肉瘤中的作用，对23个肿瘤样本进行了回顾性研究：18个原发性肿瘤以及5个化疗后复发肿瘤或二次探查手术时获取的标本。通过流式细胞术和图像细胞术对石蜡包埋组织解离后的细胞核进行DNA分析，并将其与组织学及临床病史相关联。通过流式细胞术检测，原发性肿瘤中4个为二倍体，4个为多倍体，10个为非整倍体（9个有单个非整倍体G0G1峰，1个为多倍体）。流式细胞术和图像细胞术之间的符合率为23例中的19例（83%）；1例无法进行流式细胞术检测。大多数胚胎性横纹肌肉瘤为非整倍体（12例中的10例；83%），且在Ⅲ期和Ⅳ期有较高的复发率（12例中的4例；33%）。尽管儿童癌症中的非整倍体可能预示治疗反应及良好预后，但我们在横纹肌肉瘤中的研究结果并不支持这一点。肿瘤DNA含量与临床分期相关，但与患者的临床病程或肿瘤组织病理学类型无关。DNA含量似乎并非像肿瘤分期那样重要的预后工具。
