Cer Groupe, Santés animale et humaine, Rue Point du Jour 8, 6900 Marloie, Belgium.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2009 Oct;26(10):1341-7. doi: 10.1080/02652030903013328.
A surface plasmon resonance biosensor immunoassay has been developed for multi-residue determination of 13 (fluoro)quinolone antibiotics in poultry meat, eggs and fish. The following performance characteristics were determined according to the guidelines laid down for screening assay validation in European Decision 2002/657/EC: detection capability, specificity/selectivity, decision limit, repeatability, ruggedness and stability. The detection capability estimated for norfloxacin, the reference fluoroquinolone, was below 0.5, 1 and 1.5 ng g⁻¹ for poultry meat, egg and fish, respectively. The screening assay proved specific and showed satisfactory sensitivity below the MRL levels even though flumequine and oxolinic acid had lower cross-reactivities. A wide range of non-MRL substances were also detected at concentrations below 10 ng g⁻¹. Repeatability was good with both intra- and inter-assay coefficients of variation 56%; ruggedness was also demonstrated.
已经开发出一种表面等离子体共振生物传感器免疫分析法，用于多残留测定禽肉、蛋和鱼中的 13 种（氟）喹诺酮类抗生素。根据欧洲决定 2002/657/EC 中规定的筛选分析验证准则，确定了以下性能特征：检测能力、特异性/选择性、决策限、重复性、稳健性和稳定性。对于诺氟沙星（参考氟喹诺酮类药物），分别估算出禽肉、蛋和鱼中的检测能力低于 0.5、1 和 1.5ng/g。尽管氟甲喹和恶喹酸的交叉反应性较低，但该筛选分析证明具有特异性，并且在低于 MRL 水平时表现出令人满意的灵敏度。还检测到浓度低于 10ng/g 的多种非 MRL 物质。内标和外标分析的变异系数均为 56%，重复性良好，稳健性也得到了证明。
