Center for Food Safety, Institute of Food Science & Engineering, Department of Food Science, University of Arkansas, Fayetteville, AR 72704, USA.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2009 Oct;26(10):1348-53. doi: 10.1080/02652030903013294.
In 2005, the FDA withdrew approval for the use of fluoroquinolones in live poultry production. To assess any changes in countable numbers of ciprofloxacin-resistant Campylobacter before and after the fluoroquinolone withdrawal, retail whole raw chicken carcasses (RTCC) purchased in Northwest Arkansas from 2004 to 2006 were sampled for this purpose. Using a previously published direct-plating method developed in our laboratory, we quantified trends of Campylobacter and ciprofloxacin-resistant Campylobacter loads by direct plating whole chicken carcass rinses on Campylobacter agar (CA) or Campylobacter agar containing 8.6 mg/ml ciprofloxacin (CCA). Countable populations of Campylobacter were recovered from 74, 96, and 63% of carcasses sampled in 2004, 2005, and 2006 respectively. The percentages of carcasses with minimum detectable levels of ciprofloxacin-resistant Campylobacter were 20, 42 and 33%, sampled in 2004, 2005 and 2006, respectively. Our 3 year analysis in one geographical area indicated a persistence of Campylobacter and ciprofloxacin-resistant Campylobacter on retail raw chicken carcasses despite the cessation of fluoroquinolone use in poultry production.
2005 年，FDA 撤回了在活禽生产中使用氟喹诺酮类药物的批准。为了评估氟喹诺酮类药物撤出前后计数数量的环丙沙星耐药弯曲杆菌的任何变化，为此目的从 2004 年至 2006 年在阿肯色州西北部购买零售整只生鸡（RTCC）进行了采样。使用我们实验室先前发表的直接平板法，我们通过直接在弯曲杆菌琼脂（CA）或含 8.6mg/ml 环丙沙星的弯曲杆菌琼脂（CCA）上直接平板冲洗整只鸡胴体，定量了弯曲杆菌和环丙沙星耐药弯曲杆菌负荷的趋势。在 2004 年、2005 年和 2006 年分别从 74%、96%和 63%的胴体中回收了可计数的弯曲杆菌种群。在 2004 年、2005 年和 2006 年分别采样的鸡胴体中，最小可检测水平的环丙沙星耐药弯曲杆菌的百分比分别为 20%、42%和 33%。我们在一个地理区域的 3 年分析表明，尽管停止了在禽类生产中使用氟喹诺酮类药物，但零售生鸡胴体上仍存在弯曲杆菌和环丙沙星耐药弯曲杆菌。
