Department of Cereal and Food Science, North Dakota State University , Fargo, North Dakota 58108-6050, United States.
J Agric Food Chem. 2011 May 11;59(9):4597-605. doi: 10.1021/jf104945x. Epub 2011 Apr 11.
Black soybean is known to have a health-promoting effect because of its high content of polyphenolic compounds and antioxidant activities. The objective of the present study was to investigate the chemopreventive effects of black soybean extract against human AGS gastric cancer cells and its possible mechanism in inducing apoptosis. Black soybean extract was obtained by extracting black soybean with acidified aqueous acetone, and its phytochemical constituents, as determined by HPLC-DAD methods, were demonstrated to contain various phenolics. The black soybean extract inhibited AGS cell growth in a dose-dependent manner with an IC(50) of 3.69 mg/mL as measured by the MTT assay. This growth inhibition effect was further confirmed by the CFDA-SE assay. Flow cytometry analysis showed that black soybean extract dose-dependently induced apoptosis of AGS cells. Moreover, the involvement of black soybean extract in inducing apoptosis was confirmed by the expression of Bax, Bcl-2, caspase-3, and PARP. The results of the present study indicated that black soybean extract could be used as an apoptosis inducer in AGS cells and a natural chemopreventive agent in the treatment of human gastric cancer.
黑豆因富含多酚类化合物和抗氧化活性而具有促进健康的作用。本研究旨在探讨黑豆提取物对人 AGS 胃癌细胞的化学预防作用及其诱导细胞凋亡的可能机制。通过酸化的水性丙酮提取黑豆获得黑豆提取物，并通过 HPLC-DAD 方法确定其植物化学成分含有各种酚类物质。MTT 法测定，黑豆提取物对 AGS 细胞生长的抑制作用呈剂量依赖性，IC50 为 3.69mg/mL。CFDA-SE 测定进一步证实了这种生长抑制作用。流式细胞术分析表明，黑豆提取物剂量依赖性地诱导 AGS 细胞凋亡。此外，通过 Bax、Bcl-2、caspase-3 和 PARP 的表达证实了黑豆提取物在诱导细胞凋亡中的作用。本研究结果表明，黑豆提取物可作为 AGS 细胞凋亡诱导剂和治疗人类胃癌的天然化学预防剂。
