利用酶控制和切换胶束纳米粒子的形态。
Controlling and switching the morphology of micellar nanoparticles with enzymes.
机构信息
Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA.
出版信息
J Am Chem Soc. 2011 Jun 8;133(22):8392-5. doi: 10.1021/ja2004736. Epub 2011 Apr 4.
Abstract
Micelles were prepared from polymer-peptide block copolymer amphiphiles containing substrates for protein kinase A, protein phosphatase-1, and matrix metalloproteinases 2 and 9. We examine reversible switching of the morphology of these micelles through a phosphorylation-dephosphorylation cycle and study peptide-sequence directed changes in morphology in response to proteolysis. Furthermore, the exceptional uniformity of these polymer-peptide particles makes them amenable to cryo-TEM reconstruction techniques lending insight into their internal structure.
摘要
胶束由含有蛋白激酶 A、磷酸酶-1 和基质金属蛋白酶 2 和 9 底物的聚合物-肽嵌段共聚物两亲物制备而成。我们通过磷酸化-去磷酸化循环研究这些胶束形态的可逆转换，并研究肽序列对响应蛋白水解的形态变化的定向作用。此外，这些聚合物-肽颗粒异常均匀，使其适合于低温透射电子显微镜重建技术，从而深入了解其内部结构。
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本文引用的文献
1
Next-generation peptide nanomaterials: molecular networks, interfaces and supramolecular functionality.下一代肽纳米材料：分子网络、界面和超分子功能。
Chem Soc Rev. 2010 Sep;39(9):3351-7. doi: 10.1039/c0cs00035c. Epub 2010 Jul 30.
2
Power struggles in peptide-amphiphile nanostructures.肽两亲体纳米结构中的力量博弈。
Chem Soc Rev. 2010 Sep;39(9):3434-44. doi: 10.1039/b919446k. Epub 2010 Jul 20.
3
Bioresponsive peptide-inorganic hybrid nanomaterials.生物响应性肽-无机杂化纳米材料。
Chem Soc Rev. 2010 Sep;39(9):3358-70. doi: 10.1039/b919461b. Epub 2010 Jul 2.
4
Programmable shape-shifting micelles.可编程形状转变胶束。
Angew Chem Int Ed Engl. 2010 Jul 12;49(30):5076-80. doi: 10.1002/anie.201000265.
5
Stimulus responsive peptide based materials.基于刺激响应性肽的材料。
Chem Soc Rev. 2010 Sep;39(9):3394-412. doi: 10.1039/b914342b. Epub 2010 Jun 4.
6
pH and temperature responsive polymeric micelles and polymersomes by self-assembly of poly[2-(dimethylamino)ethyl methacrylate]-b-poly(glutamic acid) double hydrophilic block copolymers.通过聚[2-（二甲氨基）乙基甲基丙烯酸酯]-b-聚（谷氨酸）两亲性嵌段共聚物的自组装制备 pH 和温度响应性聚合物胶束和聚合物囊泡。
Langmuir. 2010 Jul 6;26(13):10546-54. doi: 10.1021/la1005693.
7
Tuning supramolecular rigidity of peptide fibers through molecular structure.通过分子结构调控肽纤维的超分子刚性。
J Am Chem Soc. 2010 May 5;132(17):6041-6. doi: 10.1021/ja908560n.
8
Matrix metalloproteinases: regulators of the tumor microenvironment.基质金属蛋白酶：肿瘤微环境的调节剂。
Cell. 2010 Apr 2;141(1):52-67. doi: 10.1016/j.cell.2010.03.015.
9
Peptide-based methods for the preparation of nanostructured inorganic materials.基于肽的方法制备纳米结构无机材料。
Angew Chem Int Ed Engl. 2010 Mar 8;49(11):1924-42. doi: 10.1002/anie.200903572.
10
Thermally induced micelle to vesicle morphology transition for a charged chain end diblock copolymer.热诱导的胶束到囊泡形态转变用于荷电链端两嵌段共聚物。
Chem Commun (Camb). 2010 Feb 21;46(7):1091-3. doi: 10.1039/b922289h. Epub 2009 Dec 16.
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