[豚鼠形觉剥夺性近视视网膜中环磷酸鸟苷的表达及超微结构变化]
[Expression of cyclic guanine monophosphate and the ultrastructure change in retina of guinea pig with form-deprivation myopia].
作者信息
Wen Dan, Liu Shuangzhen, Mao Junfeng, Tan Xingping, Xia Chaohua, Yin Chunan
机构信息
Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha 410008, China.
出版信息
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2011 Mar;36(3):249-53. doi: 10.3969/j.issn.1672-7347.2011.03.010.
OBJECTIVE
To explore the expression of cyclic guanine monophosphate (cGMP) and the ultrastructure change in retina of guinea pig with form-deprivation myopia and the underlying mechanisms.
METHODS
Three-weeks-old guinea pigs were distributed in 3 groups: an untreated group (Group I), a myopia 2-weeks group (Group II) and a myopia 3-weeks group (Group III), animals underwent monocular form-deprivation by facemask for 2 and 3 weeks. The right eyes were deprived and the left eyes were self-controlled. The refraction and axial length of the eyes was measured. Retina was observed by electron microscope. The expression of cGMP was detected by radioimmunochemistry.
RESULTS
Deprived eyes in guinea pig showed significant development of myopia, the refraction and axial length was increased. The pathological changes in ultrastructure of retina were aggravated with the development of myopia. The expression of cGMP was significantly up-regulated in the deprived eyes compared with self-control eyes(P<0.05).
CONCLUSION
Form-deprivation can up-regulate the expression of cyclic GMP, which might play an important role in the development of myopia.
目的
探讨形觉剥夺性近视豚鼠视网膜中环磷酸鸟苷（cGMP）的表达及超微结构变化及其潜在机制。
方法
将3周龄豚鼠分为3组：未处理组（Ⅰ组）、近视2周组（Ⅱ组）和近视3周组（Ⅲ组），动物通过面罩进行单眼形觉剥夺2周和3周。右眼被剥夺，左眼作为自身对照。测量眼睛的屈光和眼轴长度。用电子显微镜观察视网膜。用放射免疫化学法检测cGMP的表达。
结果
豚鼠被剥夺眼出现明显的近视发展，屈光和眼轴长度增加。随着近视的发展，视网膜超微结构的病理变化加重。与自身对照眼相比，被剥夺眼中cGMP的表达明显上调（P<0.05）。
结论
形觉剥夺可上调环磷酸鸟苷的表达，这可能在近视的发生发展中起重要作用。
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