左旋多巴可抑制豚鼠形觉剥夺性近视的发展。

Levodopa inhibits the development of form-deprivation myopia in guinea pigs.

作者信息

Mao Junfeng, Liu Shuangzhen, Qin Wenjuan, Li Fengyun, Wu Xiaoying, Tan Qian

机构信息

Department of Ophthalmology, Xiang-Ya Hospital, Central South University, Changsha, Hunan, China.

出版信息

Optom Vis Sci. 2010 Jan;87(1):53-60. doi: 10.1097/OPX.0b013e3181c12b3d.

DOI:10.1097/OPX.0b013e3181c12b3d

PMID:19901858

Abstract

PURPOSE

It has been shown that visual deprivation leads to a myopic refractive error and also reduces the retinal concentration of dopamine. Exogenously 3,4-dihydroxy-L-phenylalanine (levodopa, L-DOPA) can be converted into dopamine in vivo, which safely and effectively treats Parkinson disease. Moreover, L-DOPA was also used in the treatment of amblyopia in clinical studies. However, the effect of L-DOPA on the development of myopia has not been studied. The aim of this study was to investigate whether intraperitoneal injection of L-DOPA could inhibit form-deprivation myopia in guinea pigs and to explore a new strategy for drug treatment of myopia.

METHODS

Sixty guinea pigs, at age of 4 weeks, were randomly divided into six groups: normal control, L-DOPA group, saline group, deprived group, deprived plus L-DOPA group, and deprived plus saline group. Form deprivation was induced with translucent eye shields on the right eye and lasted for 10 days. L-DOPA was injected intraperitoneally into the guinea pig once a day. The corneal radius of curvature, refraction, and axial length were measured in all animals. Subsequently, retinal dopamine content was evaluated by high-performance liquid chromatography with electrochemical detection.

RESULTS

Ten days of eye occlusion caused the form-deprived eyes to elongate and become myopic, and retinal dopamine content to decrease, but the corneal radius of curvature was not affected. Repeated intraperitoneal injection of L-DOPA could inhibit the myopic shift (from -3.62 +/- 0.98 D to -1.50 +/- 0.38 D; p < 0.001) due to goggles occluding and compensate retinal dopamine (from 0.65 +/- 0.10 ng to 1.33 +/- 0.23 ng; p < 0.001). Administration of L-DOPA to the unoccluded animals had no effect on its ocular refraction. There was no effect of intraperitoneal saline on the ocular refractive state and retinal dopamine.

CONCLUSIONS

Systemic L-DOPA was partly effective in this guinea pig model and, therefore, is worth testing for effectiveness in progressing human myopes.

摘要

目的

已有研究表明，视觉剥夺会导致近视性屈光不正，还会降低视网膜中多巴胺的浓度。外源性3,4-二羟基-L-苯丙氨酸（左旋多巴，L-DOPA）在体内可转化为多巴胺，它能安全有效地治疗帕金森病。此外，在临床研究中L-DOPA也被用于弱视的治疗。然而，L-DOPA对近视发展的影响尚未得到研究。本研究的目的是探讨腹腔注射L-DOPA是否能抑制豚鼠的形觉剥夺性近视，并探索一种新的近视药物治疗策略。

方法

60只4周龄的豚鼠随机分为六组：正常对照组、L-DOPA组、生理盐水组、剥夺组、剥夺加L-DOPA组和剥夺加生理盐水组。用半透明眼罩诱导右眼形觉剥夺，持续10天。每天给豚鼠腹腔注射一次L-DOPA。测量所有动物的角膜曲率半径、屈光和眼轴长度。随后，采用高效液相色谱电化学检测法评估视网膜多巴胺含量。

结果

10天的眼部遮盖导致形觉剥夺眼眼轴伸长并出现近视，视网膜多巴胺含量降低，但角膜曲率半径未受影响。反复腹腔注射L-DOPA可抑制因眼罩遮盖引起的近视性偏移（从-3.62±0.98 D降至-1.50±0.38 D；p<0.001），并使视网膜多巴胺得到补充（从0.65±0.10 ng升至1.33±0.23 ng；p<0.001）。给未遮盖眼睛的动物注射L-DOPA对其眼屈光无影响。腹腔注射生理盐水对眼屈光状态和视网膜多巴胺无影响。