Microsystems and BioMEMS Laboratory, Department of Electrical and Computer Engineering, University of Cincinnati, Cincinnati, OH 45221, USA.
Biomed Microdevices. 2011 Aug;13(4):661-9. doi: 10.1007/s10544-011-9536-4.
This work seeks to extend the utility of microfluidics to conventional blood sampling aperati. Daily medical care of hospitalized patients demands repeated needle punctures or interfacing with a catheter to collect blood samples. Large, research grade systems can autonomously sample blood from laboratory animals; however, a disposable aperatus that can be used to repeatedly sample blood from hospitalized patients does not exist. We have designed, fabricated and demonstrated a 3-layered rigid polymer microfluidic blood sampling device with integrated polymer pinch valves for placement in-line between a patient and a saline infusion system. The blood sampler we designed seeks to mitigate sample cross contamination, reduce risks of microbial contamination associated with invasive blood sampling and improve technical ease of blood sampling. Clinical laboratory tests and microfluidic devices for rapid point-of-care-testing (POCT) of patient samples require human sampling procedures for collection of a patient sample at defined time points. The microfluidic sampling device is designed ultimately to be backwards compatible with existing clinical saline infusion protocols and function as a universal front-end blood sampling unit for the variety of microfluidic lab chips and POCT devices.
这项工作旨在将微流控技术扩展到传统的血液采样设备中。住院患者的日常医疗护理需要反复进行针穿刺或与导管接口以采集血液样本。大型研究级系统可以自主从实验动物中采集血液样本；然而，不存在可用于从住院患者中重复采集血液样本的一次性设备。我们设计、制造并展示了一种具有集成聚合物夹管阀的 3 层刚性聚合物微流控血液采样装置，可放置在患者和盐水输注系统之间的在线位置。我们设计的血液采样器旨在减轻样本交叉污染，降低与侵入性血液采样相关的微生物污染风险，并提高血液采样的技术便利性。临床实验室测试和用于患者样本快速即时检验 (POCT) 的微流控设备需要人为采样程序，以便在规定的时间点采集患者样本。微流控采样设备最终旨在与现有的临床盐水输注方案向后兼容，并作为各种微流控实验室芯片和 POCT 设备的通用前端血液采样单元。
