Hovi T
Med Microbiol Immunol. 1978 Nov 17;166(1-4):173-6. doi: 10.1007/BF02121147.
Antibodies against the human coronavirus OC43 in human sera were measured by haemagglutination inhibition (HI), complement fixation (CF), and radial diffusion-haemolysis in gel (HIG) techniques. The apparent HI titres in a fraction of sera with no antibodies detectable by the two other methods were found to be reduced considerably after treating the sera with phospholipase C (PLC). The PLC treatment also reduced the apparent HI titres in some sera containing variable amounts of CF and/or HIG antibodies, but did not affect antibody determinations by the two latter methods. These results suggest that false positive results can be obtained in the OC43 HI test unless the sera are treated with phospholipase C before the assay.
采用血凝抑制(HI)、补体结合(CF)和凝胶中的放射扩散 - 溶血(HIG)技术检测人血清中针对人冠状病毒OC43的抗体。在用磷脂酶C(PLC)处理血清后,发现一部分用其他两种方法检测不到抗体的血清中的表观HI效价大幅降低。PLC处理还降低了一些含有不同量CF和/或HIG抗体的血清中的表观HI效价,但不影响后两种方法的抗体测定。这些结果表明,除非在检测前用磷脂酶C处理血清,否则在OC43 HI试验中可能会获得假阳性结果。