意大利人冠状病毒OC43感染的血清流行病学研究。

Seroepidemiologic study of human coronavirus OC43 infections in Italy.

作者信息

Gerna G, Cattaneo E, Cereda P, Revello M G

出版信息

Boll Ist Sieroter Milan. 1978 Sep 30;57(4):535-42.

Abstract

Human Coronavirus OC43 from suckling mouse brain (SMB) suspension has been adapted to grow in human embryonic lung fibroblast cell cultures. Complement-fixing and hemagglutinating antigens were prepared from infected cell cultures and used in parallel with the same antigens from SMB for a study of the prevalence of OC43 antibody in Northern Italy as well as for the serodiagnosis of recent OC43 infections and reinfections. Results show (i) a higher sensitivity of the hemagglutination-inhibition as compared to the complement fixation test both for antibody detection in seroepidemiological studies and serodiagnosis of acute infections, (ii) a high prevalence of OC43 antibody in Northern Italy, so that only exceptionally are people older than 12 years seronegative, (iii) a high rate of OC43 reinfections during the winter-spring season 1976/77 in a population sample with preexisting antibody (iv) a good quality of reagents prepared from cell cultures, and hence free of contaminating mouse viruses, such as mouse hepatitis virus.

摘要

来自乳鼠脑（SMB）悬液的人冠状病毒OC43已适应在人胚肺成纤维细胞培养物中生长。从感染的细胞培养物中制备补体结合抗原和血凝抗原，并与来自SMB的相同抗原并行用于研究意大利北部OC43抗体的流行情况以及近期OC43感染和再感染的血清学诊断。结果显示：（i）在血清流行病学研究中的抗体检测和急性感染的血清学诊断方面，血凝抑制试验的敏感性高于补体结合试验；（ii）意大利北部OC43抗体的高流行率，以至于12岁以上的人只有极少数血清学阴性；（iii）在1976/77年冬春季节，在已有抗体的人群样本中OC43再感染率很高；（iv）从细胞培养物中制备的试剂质量良好，因此不含诸如小鼠肝炎病毒等污染性小鼠病毒。