Donate L E, Murialdo H, Carrascosa J L
Centro de Biología Molecular (CSIC-UAM), Facultad de Ciencias, Universidad Autónoma, Madrid, Spain.
Virology. 1990 Dec;179(2):936-40. doi: 10.1016/0042-6822(90)90172-n.
Proheads of bacteriophage lambda which carry the connector of phage phi 29 instead of that of lambda have been produced in vitro. These hybrid proheads have a structure similar to that of normal lambda proheads. Furthermore, the chimeric proheads can package both lambda and phi 29 DNA. These data show that the connector domains involved in both head assembly and DNA packaging are functionally similar. The DNA-containing lambda-phi 29 proheads can be complemented in vitro with phi 29 tails to yield infective particles capable of DNA transfer.
已在体外制备了携带噬菌体φ29的连接体而非λ噬菌体连接体的λ噬菌体原头部。这些杂合原头部具有与正常λ噬菌体原头部相似的结构。此外，嵌合原头部能够包装λ噬菌体和φ29噬菌体的DNA。这些数据表明，参与头部组装和DNA包装的连接体结构域在功能上相似。含有DNA的λ-φ29原头部可在体外与φ29噬菌体尾部互补，以产生能够进行DNA转移的感染性颗粒。
