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[结构基因区域发生重组的λ-φ80杂交噬菌体的限制性内切酶BamHI、HindII、EcoRI和SmaI图谱分析]

[Restrictase BamHI, HindII, EcoRI and SmaI mapping of hybrid lambda-phi 80 phages with recombination in the structural gene region].

作者信息

Boĭtsov A S, Shaleva O N, Rybchin V N

出版信息

Genetika. 1981;17(11):1895-903.

PMID:6274737
Abstract

Hybrids lambda H lambda T80 with recombination in the region of structural genes have lambda head and phi 80 tail genes. In this paper the molecular structure of 5 independently isolated hybrids was established using restriction endonucleases. It has been shown that all of them have a recombinant head or tail. A deletion of 4,8% lambda was demonstrated in the immunity region of phi 80vir phage. Co-ordinates of restriction sites for BamHI, HindIII, EcoRI and SmaI restriction endonucleases on phi 80 DNA were calculated.

摘要

在结构基因区域发生重组的λHλT80杂种具有λ头部和φ80尾部基因。本文使用限制性内切酶确定了5个独立分离的杂种的分子结构。结果表明,它们都有重组的头部或尾部。在φ80vir噬菌体的免疫区域发现了4.8%的λ缺失。计算了φ80 DNA上BamHI、HindIII、EcoRI和SmaI限制性内切酶的限制位点坐标。

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