核心人类线粒体转录起始复合物：双人探戈只需两人。
The core human mitochondrial transcription initiation complex: It only takes two to tango.
作者信息
Shutt Timothy E, Bestwick Megan, Shadel Gerald S
机构信息
Department of Pathology Yale University School of Medicine; New Haven, CT USA.
出版信息
Transcription. 2011 Mar;2(2):55-59. doi: 10.4161/trns.2.2.14296.
Abstract
We recently demonstrated that the core transcription initiation complex in human mitochondria is a two-component system (POLRMT and h-mtTFB2). Human mtTFA/TFAM, previously proposed to be a requisite initiation complex member, is dispensable for promoter-specific initiation in vitro. We propose that it instead regulates relative promoter activity and/or overall nucleoid transcription and replication potential.
摘要
我们最近证明，人类线粒体中的核心转录起始复合物是一个双组分系统（POLRMT和h-mtTFB2）。先前被认为是必需起始复合物成员的人类mtTFA/TFAM，在体外对于启动子特异性起始是可有可无的。我们提出，它反而调节相对启动子活性和/或整体类核转录及复制潜能。
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2
Maintenance of respiratory chain function in mouse hearts with severely impaired mtDNA transcription.
Nucleic Acids Res. 2010 Oct;38(19):6577-88. doi: 10.1093/nar/gkq527. Epub 2010 Jun 20.
3
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4
A compendium of human mitochondrial gene expression machinery with links to disease.
Environ Mol Mutagen. 2010 Jun;51(5):360-79. doi: 10.1002/em.20571.
5
Human mitochondrial transcription revisited: only TFAM and TFB2M are required for transcription of the mitochondrial genes in vitro.
J Biol Chem. 2010 Jun 11;285(24):18129-33. doi: 10.1074/jbc.C110.128918. Epub 2010 Apr 21.
6
TFB2 is a transient component of the catalytic site of the human mitochondrial RNA polymerase.
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DNA conformation-dependent activities of human mitochondrial RNA polymerase.
Genes Cells. 2009 Aug;14(8):1029-42. doi: 10.1111/j.1365-2443.2009.01328.x. Epub 2009 Jul 14.
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9
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