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经丙烯酰胺基叶酸修饰的磁性纳米粒子与癌细胞的相互作用。

Interaction of cancer cells with magnetic nanoparticles modified by methacrylamido-folic acid.

机构信息

Department of Pharmaceutical Botanics, Faculty of Pharmacy, Anadolu University, Eskişehir, Turkey.

出版信息

Int J Nanomedicine. 2011;6:477-84. doi: 10.2147/IJN.S16803. Epub 2011 Feb 28.

DOI:10.2147/IJN.S16803
PMID:21468352
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3065795/
Abstract

BACKGROUND

Magnetic nanoparticles show great promise for use as tools in a wide variety of biomedical applications. The purpose of this study was to investigate the potential effects of methacrylamido-folic acid (Ma-Fol)-modified magnetic nanoparticles on 5RP7 (H-ras-transformed rat embryonic fibroblasts) and NIH/3T3 (normal mouse embryonic fibroblasts).

METHODS

The cytotoxicity and viability of 5RP7 and NIH/3T3 cells were detected. The percentage of cells undergoing apoptosis was analyzed by flow cytometry using Annexin V-fluorescein isothiocyanate staining. Nanoparticle internalization into 5RP7 and NIH/3T3 cells was visualized by transmission electron microscopy.

CONCLUSION

In this study, folic acid coupled to the surface of iron oxide for selective binding to cancer cells and immobilized the surfaces of magnetic nanoparticles. This complex improves cell internalization and targeting of cancer cells. We detected increased apoptosis using flow cytometry and transmission electron microscopy.

RESULTS

Folic acid modification of magnetic nanoparticles could be used to facilitate uptake to specific cancer cells for cancer therapy and diagnosis. Our results showed that the uptake of folic-acid modified nanoparticles by 5RP7 cancer cells was also much higher than that of 3T3 cells. This modification can be used for successful targeting of cancer cells expressing the folate receptor.

摘要

背景

磁性纳米粒子在各种生物医学应用中作为工具具有很大的应用潜力。本研究旨在探讨甲丙烯酰胺叶酸（Ma-Fol）修饰的磁性纳米粒子对 5RP7（H-ras 转化的大鼠胚胎成纤维细胞）和 NIH/3T3（正常小鼠胚胎成纤维细胞）的潜在影响。

方法

检测 5RP7 和 NIH/3T3 细胞的细胞毒性和活力。用 Annexin V-异硫氰酸荧光素染色通过流式细胞术分析细胞凋亡的百分比。用透射电子显微镜观察纳米粒子进入 5RP7 和 NIH/3T3 细胞的情况。

结论

在这项研究中，叶酸与氧化铁表面偶联以选择性结合癌细胞，并固定磁性纳米粒子的表面。这种复合物提高了癌细胞的内化和靶向能力。我们通过流式细胞术和透射电子显微镜检测到细胞凋亡增加。

结果

磁性纳米粒子的叶酸修饰可用于促进特定癌细胞的摄取，以用于癌症治疗和诊断。我们的结果表明，叶酸修饰的纳米粒子被 5RP7 癌细胞摄取的量也远高于 3T3 细胞。这种修饰可用于成功靶向表达叶酸受体的癌细胞。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e9a/3065795/66b2b7d26fb3/ijn-6-477f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e9a/3065795/3f0c981570d4/ijn-6-477f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e9a/3065795/be251c101895/ijn-6-477f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e9a/3065795/2586d3bf0d43/ijn-6-477f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e9a/3065795/d3852335338d/ijn-6-477f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e9a/3065795/71d750d970a3/ijn-6-477f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e9a/3065795/203f51f2bc76/ijn-6-477f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e9a/3065795/66b2b7d26fb3/ijn-6-477f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e9a/3065795/3f0c981570d4/ijn-6-477f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e9a/3065795/be251c101895/ijn-6-477f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e9a/3065795/2586d3bf0d43/ijn-6-477f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e9a/3065795/d3852335338d/ijn-6-477f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e9a/3065795/71d750d970a3/ijn-6-477f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e9a/3065795/203f51f2bc76/ijn-6-477f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e9a/3065795/66b2b7d26fb3/ijn-6-477f7.jpg

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本文引用的文献

1
Nanoparticles as biochemical sensors.作为生化传感器的纳米颗粒。
Nanotechnol Sci Appl. 2010 Sep 23;3:65-76. doi: 10.2147/NSA.S8199.
2
Flow cytometric analysis on tri-n-butyltin-induced increase in annexin V binding to membranes of rat thymocytes.三丁基锡诱导大鼠胸腺细胞膜结合 Annexin V 增加的流式细胞分析。
Environ Toxicol Pharmacol. 1999 Oct;7(4):267-73. doi: 10.1016/s1382-6689(99)00026-5.
3
Magnetic Fe₃O₄ nanoparticles and chemotherapy agents interact synergistically to induce apoptosis in lymphoma cells.磁性 Fe₃O₄ 纳米粒子与化疗药物协同作用诱导淋巴瘤细胞凋亡。
基于纳米技术和绿色化学的药物递送系统综述：绿色纳米医学
Int J Nanomedicine. 2017 Apr 12;12:2957-2978. doi: 10.2147/IJN.S127683. eCollection 2017.
4
Increased cellular uptake of lauryl gallate loaded in superparamagnetic poly(methyl methacrylate) nanoparticles due to surface modification with folic acid.由于用叶酸进行表面修饰，超顺磁性聚甲基丙烯酸甲酯纳米颗粒中负载的没食子酸月桂酯的细胞摄取增加。
J Mater Sci Mater Med. 2016 Dec;27(12):185. doi: 10.1007/s10856-016-5796-0. Epub 2016 Oct 27.
5
Apoptosis selectively induced in BEL-7402 cells by folic acid-modified magnetic nanoparticles combined with 100 Hz magnetic field.叶酸修饰的磁性纳米颗粒联合100Hz磁场在BEL-7402细胞中选择性诱导凋亡。
Int J Nanomedicine. 2014 Apr 23;9:2043-50. doi: 10.2147/IJN.S60457. eCollection 2014.
6
Cytotoxicity evaluation of carbon-encapsulated iron nanoparticles in melanoma cells and dermal fibroblasts.碳包封铁纳米颗粒对黑色素瘤细胞和真皮成纤维细胞的细胞毒性评估
J Nanopart Res. 2013;15(8):1835. doi: 10.1007/s11051-013-1835-7. Epub 2013 Jul 24.
7
Immune response to nanomaterials: implications for medicine and literature review.免疫对纳米材料的反应：对医学的影响和文献综述。
Curr Allergy Asthma Rep. 2013 Feb;13(1):50-7. doi: 10.1007/s11882-012-0302-3.
8
Phagocytes mediate targeting of iron oxide nanoparticles to tumors for cancer therapy.吞噬细胞介导氧化铁纳米颗粒靶向肿瘤用于癌症治疗。
Integr Biol (Camb). 2013 Jan;5(1):159-71. doi: 10.1039/c2ib20180a.
9
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Int J Nanomedicine. 2012;7:1903-20. doi: 10.2147/IJN.S29442. Epub 2012 Apr 18.
10
Expression and preliminary functional analysis of Siglec-F on mouse macrophages.Siglec-F 在小鼠巨噬细胞上的表达及初步功能分析。
J Zhejiang Univ Sci B. 2012 May;13(5):386-94. doi: 10.1631/jzus.B1100218.
Int J Nanomedicine. 2010 Nov 19;5:999-1004. doi: 10.2147/IJN.S14957.
4
Biocompatibility of Fe3O4/DNR magnetic nanoparticles in the treatment of hematologic malignancies.Fe3O4/DNR 磁性纳米粒子治疗血液系统恶性肿瘤的生物相容性。
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5
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6
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7
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8
On the chemical synthesis and drug delivery response of folate receptor-activated, polyethylene glycol-functionalized magnetite nanoparticles.关于叶酸受体激活的聚乙二醇功能化磁铁矿纳米颗粒的化学合成及药物递送响应
Acta Biomater. 2008 Jan;4(1):40-8. doi: 10.1016/j.actbio.2007.06.006. Epub 2007 Jul 1.
9
Inhibition of mammalian target of rapamycin or apoptotic pathway induces autophagy and radiosensitizes PTEN null prostate cancer cells.抑制雷帕霉素哺乳动物靶点或凋亡途径可诱导自噬并使PTEN缺失的前列腺癌细胞对放疗敏感。
Cancer Res. 2006 Oct 15;66(20):10040-7. doi: 10.1158/0008-5472.CAN-06-0802.
10
Surface functionalization of gold nanoparticles using hetero-bifunctional poly(ethylene glycol) spacer for intracellular tracking and delivery.使用异双功能聚乙二醇间隔物对金纳米颗粒进行表面功能化以用于细胞内追踪和递送。
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