犬瘟热病毒利用不同的受体感染鸡胚成纤维细胞和vero 细胞。
Canine distemper virus utilizes different receptors to infect chicken embryo fibroblasts and vero cells.
机构信息
College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China.
出版信息
Virol Sin. 2011 Apr;26(2):139-45. doi: 10.1007/s12250-011-3176-3. Epub 2011 Apr 7.
Inducing animal viruses to adapt to chicken embryos or chicken embryo fibroblasts (CEF) is a common method to develop attenuated live vaccines with full security. Canine distemper virus (CDV) also does this, but the mechanisms and particular receptors remain unclear. Virus overlay protein blot assays were carried out on CEF membrane proteins, which were extracted respectively with a Mem-PER™ kit, a radioimmunoprecipitation assay buffer or a modified co-immunoprecipitation method, and revealed a common 57 kDa positive band that differed from the 42-kDa positive band in Vero cells and also from those receptors reported in lymphocytes and 293 cells, indicating a receptor diversity of CDV and the possibility of the 57-kDa protein acting as a receptor that is involved in adaptive infection of CDV Kunming strain to CEF.
将动物病毒诱导适应鸡胚或鸡胚成纤维细胞（CEF）是开发具有完全安全性的减毒活疫苗的常用方法。犬瘟热病毒（CDV）也是如此，但机制和特定受体仍不清楚。使用 Mem-PER™试剂盒、放射免疫沉淀测定缓冲液或改良的共免疫沉淀法分别提取 CEF 膜蛋白，并进行病毒覆盖蛋白印迹分析，揭示了一个共同的 57 kDa 阳性条带，与 Vero 细胞中的 42 kDa 阳性条带不同，也与淋巴细胞和 293 细胞中报道的那些受体不同，表明 CDV 的受体多样性和 57 kDa 蛋白作为参与 CDV 昆明株适应感染 CEF 的受体的可能性。
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