麻疹病毒对鸡胚成纤维细胞的感染：病毒进入水平的适应性
Infection of chicken embryonic fibroblasts by measles virus: adaptation at the virus entry level.
作者信息
Escoffier C, Gerlier D
机构信息
Immunité & Infections Virales, IVMC, CNRS-UCBL UMR 5537, 69372 Lyon Cedex 08, France.
出版信息
J Virol. 1999 Jun;73(6):5220-4. doi: 10.1128/JVI.73.6.5220-5224.1999.
Abstract
Measles virus (MV) has a tropism restricted to humans and primates and uses the human CD46 molecule as a cellular receptor. MV has been adapted to grow in chicken embryonic fibroblasts (CEF) and gave rise to an attenuated live vaccine. Hallé and Schwarz MV strains were compared in their ability to infect both simian Vero cells and CEF. Whereas both strains infected Vero cells, only the CEF-adapted Schwarz strain was able to efficiently infect CEF. Since the expression of the human MV receptor CD46 rendered the chicken embryonic cell line TCF more permissive to the infection by the Hallé MV strain, the MV entry into CEF appeared to be a limiting step in the absence of prior MV adaptation. CEF lacked reactivity with anti-CD46 antibodies but were found to express another protein allowing MV binding as an alternative receptor to CD46.
摘要
麻疹病毒（MV）具有仅限于人类和灵长类动物的嗜性，并将人类CD46分子用作细胞受体。MV已被适应在鸡胚成纤维细胞（CEF）中生长，并由此产生了一种减毒活疫苗。对哈勒（Hallé）株和施瓦茨（Schwarz）株感染猿猴肾Vero细胞和CEF的能力进行了比较。虽然两种毒株都能感染Vero细胞，但只有适应CEF的施瓦茨毒株能够有效感染CEF。由于人类MV受体CD46的表达使鸡胚细胞系TCF对哈勒MV毒株的感染更具易感性，因此在没有事先对MV进行适应的情况下，MV进入CEF似乎是一个限制步骤。CEF与抗CD46抗体无反应，但发现其表达另一种蛋白质，可作为CD46的替代受体允许MV结合。
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