Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA.
Annu Rev Anal Chem (Palo Alto Calif). 2011;4:419-37. doi: 10.1146/annurev.anchem.111808.073722.
Second-order nonlinear optical imaging of chiral crystals (SONICC) is an emerging technique for crystal imaging and characterization. We provide a brief overview of the origin of second harmonic generation signals in SONICC and discuss recent studies using SONICC for biological applications. Given that they provide near-complete suppression of any background, SONICC images can be used to determine the presence or absence of protein crystals through both manual inspection and automated analysis. Because SONICC creates high-resolution images, nucleation and growth kinetics can also be observed. SONICC can detect metastable, homochiral crystalline forms of amino acids crystallizing from racemic solutions, which confirms Ostwald's rule of stages for crystal growth. SONICC's selectivity, based on order, and sensitivity, based on background suppression, make it a promising technique for numerous fields concerned with chiral crystal formation.
手性晶体的二阶非线性光学成像(SONICC)是一种新兴的晶体成像和表征技术。我们简要概述了 SONICC 中二次谐波产生信号的起源,并讨论了最近使用 SONICC 进行生物应用的研究。由于 SONICC 可以完全抑制任何背景,因此 SONICC 图像可用于通过手动检查和自动分析来确定蛋白质晶体的存在与否。由于 SONICC 可以创建高分辨率图像,因此还可以观察成核和生长动力学。SONICC 可以检测从外消旋溶液中结晶的氨基酸的亚稳态、同手性晶体形态,这证实了奥斯特瓦尔德阶段规则对晶体生长的影响。基于有序的选择性和基于背景抑制的灵敏度使 SONICC 成为许多关注手性晶体形成的领域的有前途的技术。