时间分辨连续晶体学的样品制备：实用考虑因素。
Sample Preparation for Time-Resolved Serial Crystallography: Practical Considerations.
机构信息
Institute for Nanostructure and Solid State Physics, Universität Hamburg, Hamburg, Germany.
Max Planck Institute for Structure and Dynamics of Matter, Hamburg, Germany.
出版信息
Methods Mol Biol. 2023;2652:361-379. doi: 10.1007/978-1-0716-3147-8_21.
Time-resolved serial crystallography is an emerging method to elucidate the structure-function relationship of biomolecular systems at up to atomic resolution. However, to make this demanding method a success, a number of experimental requirements have to be met. In this chapter, we summarize general guidelines and protocols towards performing time-resolved crystallography experiments, with a particular emphasis on sample requirements and preparation but also a brief excursion into reaction initiation.
时间分辨连续晶体学是一种新兴的方法，可在原子分辨率水平上阐明生物分子系统的结构-功能关系。然而，要成功应用这种要求苛刻的方法，需要满足许多实验要求。在本章中，我们总结了进行时间分辨晶体学实验的一般指南和方案，特别强调了样品要求和准备，但也简要介绍了反应引发。
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