Biomedical Research Centre, Sheffield Hallam University, City Campus, Sheffield S1 1WB, UK.
Hum Reprod. 2011 Jun;26(6):1331-7. doi: 10.1093/humrep/der104. Epub 2011 Apr 6.
CD56+ cells in peripheral blood or the endometrium may be increased in women with reproductive failure. However, the relationship between numbers of peripheral blood CD56+ and endometrial CD56+ cells is uncertain. The aim of this study was (i) to compare the numbers of CD56+ cells in peripheral blood and endometrium in samples taken simultaneously and (ii) to compare measurements by flow cytometry and immunohistochemistry of CD56+ cells in the same endometrial biopsy.
Endometrial biopsies and blood were obtained from women with recurrent miscarriage (n= 25) on days LH+7-LH+9 of the cycle. The total number of CD56+, CD56+ CD16- and CD56+CD16+ cells in blood was measured by flow cytometry; the number of CD56+ cells in the endometrium was determined by immunohistochemistry. Endometrial samples were also obtained from fertile women (n= 20) and used to measure CD56+ and CD45+ cells, by both flow cytometry and immunostaining.
There was no correlation between the numbers of total CD56+, CD56+CD16- or CD56+CD16+ in peripheral blood and the number of endometrial CD56+ cells in the same women. In endometrium from fertile women, a significant correlation was found between the numbers of CD56+ cells measured by flow cytometry and immunohistochemistry (correlation= 0.497, P= 0.026, when expressed as % total cells; correlation= 0.570, P= 0.009 when expressed as % CD45+ cells).
Measurements of CD56+ cells in peripheral blood do not correlate with endometrial CD56+ cell numbers and therefore should not be extrapolated to events in the endometrium. In contrast, measurements of endometrial CD56+ cells by flow cytometry and immunostaining correlate well.
外周血或子宫内膜中 CD56+细胞可能在生殖失败的女性中增加。然而，外周血 CD56+细胞数量与子宫内膜 CD56+细胞之间的关系尚不确定。本研究的目的是（i）比较同时采集的外周血和子宫内膜样本中 CD56+细胞的数量，（ii）比较同一子宫内膜活检中 CD56+细胞的流式细胞术和免疫组织化学测量。
在周期 LH+7-LH+9 天，从反复流产的女性（n=25）中获取子宫内膜活检和血液。通过流式细胞术测量外周血中 CD56+、CD56+CD16-和 CD56+CD16+细胞的总数；通过免疫组织化学测定子宫内膜中 CD56+细胞的数量。还从生育能力正常的女性（n=20）中获取子宫内膜样本，并用流式细胞术和免疫染色法测量 CD56+和 CD45+细胞。
同一女性外周血中总 CD56+、CD56+CD16-或 CD56+CD16+细胞数量与子宫内膜 CD56+细胞数量之间无相关性。在生育能力正常的女性子宫内膜中，流式细胞术和免疫组织化学测量的 CD56+细胞数量之间存在显著相关性（以总细胞的百分比表示时，相关性=0.497，P=0.026；以 CD45+细胞的百分比表示时，相关性=0.570，P=0.009）。
外周血中 CD56+细胞的测量值与子宫内膜 CD56+细胞数量不相关，因此不应推断为子宫内膜的事件。相比之下，流式细胞术和免疫组织化学测量的子宫内膜 CD56+细胞测量值相关性较好。
