电子断层扫描揭示多角体蛋白的结合以及在感染性多角体内部存在空和满的细胞质多角体病毒粒子。
Electron tomography reveals polyhedrin binding and existence of both empty and full cytoplasmic polyhedrosis virus particles inside infectious polyhedra.
机构信息
Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095, USA.
出版信息
J Virol. 2011 Jun;85(12):6077-81. doi: 10.1128/JVI.00103-11. Epub 2011 Apr 6.
Abstract
Previous studies have described the structure of purified cytoplasmic polyhedrosis virus (CPV) and that of polyhedrin protein. However, how polyhedrin molecules embed CPV particles inside infectious polyhedra is not known. By using electron tomography, we show that CPV particles are occluded within the polyhedrin crystalline lattice with a random spatial distribution and interact with the polyhedrin protein through the A-spike rather than as previously thought through the B-spike. Furthermore, both full (with RNA) and empty (no RNA) capsids were found inside polyhedra, suggesting a spontaneous RNA encapsidating process for CPV assembly in vivo.
摘要
先前的研究已经描述了纯化的细胞质多角体病毒 (CPV) 和多角体蛋白的结构。然而，多角体蛋白分子如何将 CPV 颗粒嵌入传染性多角体内部尚不清楚。通过使用电子断层扫描，我们表明 CPV 颗粒随机分布在多角体蛋白晶格内被包被，并且通过 A-刺突与多角体蛋白相互作用，而不是像以前认为的那样通过 B-刺突。此外，在多角体内部发现了完整的（带有 RNA）和空的（无 RNA）衣壳，表明 CPV 组装在体内存在自发的 RNA 包封过程。
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