Riva Alessandro, Tandler Bernard, Ushiki Tatsuo, Usai Paolo, Isola Raffaella, Conti Gabriele, Loy Francesco, L Hoppel Charles
Department of Cytomorphology, School of Medicine, University of Cagliari, Italy.
Arch Histol Cytol. 2010;73(1):37-44. doi: 10.1679/aohc.73.37.
The three-dimensional ultrastructure of over 1000 mitochondria in human Leydig cells (from twelve sexually mature patients) was examined by high resolution scanning electron microscopy (HRSEM) of osmium-macerated specimens, as well as by transmission electron microscopy of conventional ultrathin sections. The stereo-pair imaging of the osmium-macerated specimens by HRSEM is also very useful for investigating the three-dimensional structure of cytoplasmic membranous organelles with great clarity. The mitochondria, which mainly are elongated (although some are ovate), possess cristae that are almost exclusively tubular and that occasionally display constrictions and terminal bulbules. Lamelliform cristae are quite rare. Occasionally, the tubular cristae are joined together to form a simple network. Classic crista junctions could not be identified with certainty, although the base of the tubular cristae might correspond functionally to such junctions. As a whole, in line with the identical and common embryological origin of adrenal cortex and gonads, mitochondria of human Leydig cell closely resemble those of steroidogenic cells of human suprarenal cortex treated by the same maceration method.
通过对锇浸渍标本的高分辨率扫描电子显微镜（HRSEM）以及传统超薄切片的透射电子显微镜，研究了来自12名性成熟患者的人类睾丸间质细胞中1000多个线粒体的三维超微结构。HRSEM对锇浸渍标本的立体对成像对于清晰地研究细胞质膜性细胞器的三维结构也非常有用。线粒体主要呈细长形（尽管有些是卵形），其嵴几乎全为管状，偶尔可见收缩和末端小球。板层状嵴非常罕见。偶尔，管状嵴会连接在一起形成一个简单的网络。虽然管状嵴的基部在功能上可能与经典嵴连接相对应，但无法确定地识别出经典嵴连接。总体而言，鉴于肾上腺皮质和性腺具有相同且共同的胚胎学起源，人类睾丸间质细胞的线粒体与采用相同浸渍方法处理的人类肾上腺皮质类固醇生成细胞的线粒体非常相似。
