Riva Alessandro, Loffredo Felice, Uccheddu Alessandro, Riva Francesca Testa, Tandler Bernard
Department of Cytomorphology, University of Cagliari, Italy.
J Clin Endocrinol Metab. 2003 Apr;88(4):1903-6. doi: 10.1210/jc.2002-030013.
By taking advantage of a modified osmium maceration technique, we have been able to examine by high resolution scanning electron microscopy (HRSEM) the interior of human adrenocortical mitochondria from which all soluble material has been extracted. The so-called vesicles apparent in thin sections examined by transmission electron microscopy actually are finger-like cristae as determined by HRSEM. These digitiform cristae have a segmented appearance and a bulbous tip. The segmented form of the cristae may have important metabolic implications.
通过利用改良的锇浸渍技术，我们得以借助高分辨率扫描电子显微镜（HRSEM）检查已提取所有可溶性物质的人肾上腺皮质线粒体内部。透射电子显微镜检查的薄切片中出现的所谓囊泡，经HRSEM确定实际上是指状嵴。这些指状嵴具有分段外观和球状尖端。嵴的分段形式可能具有重要的代谢意义。
