Okugawa K, Fukuda Y, Sakimoto H, Nishihara M, Tashiro H, Urushihara T, Ikeda M, Iwata H, Dohi K
Second Department of Surgery, Hiroshima University School of Medicine, Japan.
Cell Transplant. 1996 Sep-Oct;5(5 Suppl 1):S51-3. doi: 10.1016/0963-6897(96)00040-1.
Two critical issues need to be addressed regarding islet cell transplantation: obtaining sufficient supply of cells for implantation, and the maintenance of the viability of these cells. Our present study has two protocols. One is islet cell implantation under the renal capsule, and the other, repeated injection of islet cells into the peritoneal cavity. These two methods were compared in an isogeneic transplant model in the rat to determine the more clinically beneficial method. Transplantation of 2000-2500 islet under the capsule of the kidney resulted in normalized blood sugar levels for more than 100 days in four of five rats with hyperglycemia. However, normalization for the same duration by islet cell injection into the peritoneal cavity necessitated repetition of injections in two out of three tested rats. In view of the similarity of the results obtained with these two protocols, intraperitoneal implantation of a few cells is preferable, because the need for prolonged cell preservation is avoided.
获取足够数量的细胞用于植入，以及维持这些细胞的活力。我们目前的研究有两种方案。一种是将胰岛细胞植入肾被膜下，另一种是将胰岛细胞反复注入腹腔。在大鼠同基因移植模型中对这两种方法进行了比较，以确定哪种方法对临床更有益。将2000 - 2500个胰岛移植到肾被膜下，五只高血糖大鼠中有四只血糖水平正常化超过100天。然而，通过向腹腔注射胰岛细胞使血糖在相同持续时间内正常化，三只受试大鼠中有两只需要重复注射。鉴于这两种方案获得的结果相似，腹腔内植入少量细胞更可取，因为避免了对细胞进行长期保存的需求。
