Katagata Yohtaro, Sasaki Fumiyuki
Hirosaki University, Faculty of Agriculture and Life Science, Department of Biochemistry and Biotechnology, Aomori 036-8561, Japan.
Mol Med Rep. 2010 Jan-Feb;3(1):179-84. doi: 10.3892/mmr_00000237.
The present study investigated the growth of human fibrosarcoma (HT-1080) and fibroblast (SF-TY) cells in combination with water-soluble (WS) and high molecular component (HMC) fractions prepared from Reishi (R), Rokkaku-Reishi (2R) and Apple Rokkaku-Reishi (A2R). Each WS fraction exhibited dose-and time-dependent inhibition of the growth of the HT-1080 and SF-TY cells. The extracts exhibited marked antiproliferative activity against the HT-1080 cells. The HMC fractions inhibited cell growth dose-and time-dependently in the HT-1080 cells only, and not in the SF-TY cells, suggesting that HMC fractions selectively inhibit HT-1080 cells. Among the HMC fractions, A2R is a strong candidate for anti-tumor targeting since its fraction exhibited better inhibition than the R and 2R fractions. Furthermore, the volume of the A2R fraction was approximately five times greater than that of the others, and included four proteins (molecular mass 9, 13, 22 and 40 kDa) detected by SDS-PAGE. Three of these (13, 22 and 40 kDa) were confirmed to be glycosylated with the Periodic Acid-Schiff Stain kit. These results suggest that A2R may possess anti-tumor activity and, in particular, that the protein components of A2R may act to selectively inhibit the growth of HT-1080 cells.
本研究调查了人纤维肉瘤（HT - 1080）和成纤维细胞（SF - TY）与从灵芝（R）、六角灵芝（2R）和苹果六角灵芝（A2R）中制备的水溶性（WS）和高分子成分（HMC）级分组合培养时的生长情况。每个WS级分均呈现出对HT - 1080和SF - TY细胞生长的剂量和时间依赖性抑制。提取物对HT - 1080细胞表现出显著的抗增殖活性。HMC级分仅对HT - 1080细胞的生长呈现剂量和时间依赖性抑制，而对SF - TY细胞无此作用，这表明HMC级分可选择性抑制HT - 1080细胞。在HMC级分中，A2R是抗肿瘤靶向的有力候选者，因为其级分表现出比R和2R级分更好的抑制作用。此外，A2R级分的体积约为其他级分的五倍，并且通过SDS - PAGE检测到其中包含四种蛋白质（分子量分别为9、13、22和40 kDa）。使用过碘酸希夫染色试剂盒证实其中三种（13、22和40 kDa）为糖基化蛋白。这些结果表明A2R可能具有抗肿瘤活性，特别是A2R的蛋白质成分可能起到选择性抑制HT - 1080细胞生长的作用。
