Kwon O-Chul, Lee Chang-Soo, Park Young-Jin
Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, Rural Development Administration, Wanju-gun 565-851, Korea.
Department of Integrated Biosciences, Research Institute for Biomedical & Health Science, College of Biomedical and Health Science, Konkuk University, Chungju-si 27478, Korea.
Microorganisms. 2019 Jan 9;7(1):12. doi: 10.3390/microorganisms7010012.
In this study we identified single nucleotide polymorphism (SNP) and sequence characteristic amplification region (SCAR) markers for specific identification of antler-shaped strains. When the partial mitochondrial SSU rDNA gene sequence of various antler- and kidney-shaped strains were analyzed and aligned, an SNP was found only in the antler-shaped strain at position 456 bp. In addition, this SNP of antler-shaped strains was digested by restriction enzyme. We further analyzed the polymorphism of various strains by random amplified polymorphic DNA (RAPD) analysis. In RAPD analysis, we isolated and sequenced a fragment, specific for antler-shaped strains. Based on this specific fragment sequence, two sets of specific primer pairs for antler-shaped strains were designed. PCR analysis revealed that two specific bands were observed only from antler-shaped strains. These two molecular markers will be helpful for identification of morphological characteristics of .
在本研究中，我们鉴定了用于特异性识别鹿角形菌株的单核苷酸多态性（SNP）和序列特征扩增区域（SCAR）标记。当分析并比对各种鹿角形和肾形菌株的部分线粒体小亚基核糖体DNA（SSU rDNA）基因序列时，仅在鹿角形菌株的456 bp位置发现了一个SNP。此外，鹿角形菌株的这个SNP可被限制性酶消化。我们通过随机扩增多态性DNA（RAPD）分析进一步分析了各种菌株的多态性。在RAPD分析中，我们分离并测序了一个对鹿角形菌株特异的片段。基于该特异性片段序列，设计了两组针对鹿角形菌株的特异性引物对。PCR分析显示，仅从鹿角形菌株中观察到两条特异性条带。这两个分子标记将有助于鉴定……的形态特征。
