Meek K, Rathbun G, Reininger L, Jaton J C, Kofler R, Tucker P W, Capra J D
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235.
Mol Immunol. 1990 Nov;27(11):1073-81. doi: 10.1016/0161-5890(90)90095-h.
During B cell development, there is an ordered expression of heavy chain variable region (VH) genes during ontogeny such that JH proximal VH genes are rearranged and expressed before the more JH distal VH genes. Thus, the relative chromosomal position of VH genes is biologically significant. We have previously employed deletion mapping to order the nine described murine VH gene families as follows: 3609-J558-(J606/VGAM3-8/S107)-3660-(X24/Q52/7183 ). (Families within parentheses were not mapped relative to each other.) In this report we continue this analysis by mapping two recently described heavy chain variable region gene families (VH10 and VH11). VH10 is located at the JH proximal end of the major cluster of J558 VH gene segments. VH11 (a very small family) is intermingled with the 3660 family. Although in general VH genes are thought to be clustered, we and others have reported some interspersion between families. To further address this issue, we have analyzed 80 recombinant phage clones containing J558 VH gene segments for the presence of other VH family genes. Our data indicate that the J558 and 3609 VH families are extensively intermingled as has recently been described for the most JH proximal Q52 and 7183 families.
在B细胞发育过程中，重链可变区（VH）基因在个体发育过程中有一个有序的表达，使得靠近JH的VH基因在更远离JH的VH基因之前发生重排和表达。因此，VH基因的相对染色体位置具有生物学意义。我们之前采用缺失作图法对九个已描述的小鼠VH基因家族进行了排序，结果如下：3609-J558-（J606/VGAM3-8/S107）-3660-（X24/Q52/7183）。（括号内的家族之间未相互定位。）在本报告中，我们通过对两个最近描述的重链可变区基因家族（VH10和VH11）进行定位来继续这项分析。VH10位于J558 VH基因片段主要簇的JH近端。VH11（一个非常小的家族）与3660家族相互交织。虽然一般认为VH基因是成簇的，但我们和其他人都报告了家族之间存在一些分散情况。为了进一步解决这个问题，我们分析了80个含有J558 VH基因片段的重组噬菌体克隆，以检测其他VH家族基因的存在。我们的数据表明，J558和3609 VH家族广泛交织，这与最近对最靠近JH的Q52和7183家族的描述一致。
