Riley S C, Connors S J, Klinman N R, Ogata R T
Proc Natl Acad Sci U S A. 1986 Apr;83(8):2589-93. doi: 10.1073/pnas.83.8.2589.
The B-cell repertoire in neonatal mice contains predominant clonotypes that are reproducibly expressed at particular times after birth. We have isolated and sequenced heavy and light chain cDNA clones from three 2,4-dinitrophenyl-specific neonatal hybridomas. Two of these hybridomas (TF2-36 and TF5-139) express idiotypes (Ids) that predominate during the first days after birth, and the third hybridoma (TF2-76) expresses an Id that predominates during the second week after birth. The heavy (H) chain variable (V) region of the TF2-76 hybridoma protein is encoded by a member of the 7183 VH family, one of eight families of murine VH genes that have been defined by Brodeur and Riblet [Brodeur, P. H. & Riblet, R. (1984) Eur. J. Immunol. 14, 922-930]. Members of this family have been found to undergo a disproportionately high frequency of rearrangement in fetal and neonatal liver pre-B-cells. Because the 7183 VH family is located close to the H chain joining (J) region gene segments, JH, other workers have proposed that VH rearrangement frequency is related to distance from the JH segments. However, the two earlier-appearing predominant clonotypes expressed by TF2-36 and TF5-139 hybridoma proteins utilize a member of the 36-60 VH family, probably VH 1210.7, which is located distal to the JH gene segments on chromosome 12. Since 20-30% of day 3 dinitrophenyl-specific B cells express either the Id(TF2-36) or the Id(TF5-139), the VH 1210.7 gene must be utilized at high frequency early in development. These results indicate that the utilization of rearranged VH segments is strongly influenced by factors other than distance from JH.
新生小鼠的B细胞库包含在出生后特定时间可重复表达的主要克隆型。我们从三个2,4 - 二硝基苯基特异性新生杂交瘤中分离并测序了重链和轻链cDNA克隆。其中两个杂交瘤（TF2 - 36和TF5 - 139）表达在出生后最初几天占主导地位的独特型（Ids），第三个杂交瘤（TF2 - 76）表达在出生后第二周占主导地位的独特型。TF2 - 76杂交瘤蛋白的重链（H）可变（V）区由7183 VH家族的一个成员编码，7183 VH家族是由布罗德和里布利特定义的八个小鼠VH基因家族之一[布罗德，P. H. & 里布利特，R.（1984年）《欧洲免疫学杂志》14卷，922 - 930页]。已发现该家族成员在胎儿和新生肝脏前B细胞中经历不成比例的高频重排。由于7183 VH家族位于重链连接（J）区基因片段JH附近，其他研究人员提出VH重排频率与距JH片段的距离有关。然而，TF2 - 36和TF5 - 139杂交瘤蛋白表达的两个较早出现的主要克隆型利用的是36 - 60 VH家族的一个成员，可能是VH 1210.7，它位于12号染色体上JH基因片段的远端。由于第3天的2,4 - 二硝基苯基特异性B细胞中有20 - 30%表达Id（TF2 - 36）或Id（TF5 - 139），VH 1210.7基因在发育早期必须被高频利用。这些结果表明，重排VH片段的利用受到距JH距离以外的因素的强烈影响。
