Rathbun G A, Capra J D, Tucker P W
Department of Microbiology, University of Texas Health Science Center, Dallas 75235.
EMBO J. 1987 Oct;6(10):2931-7. doi: 10.1002/j.1460-2075.1987.tb02597.x.
Deletion mapping analyses have been employed to order the heavy chain variable region (VH) gene families in three inbred murine strains. These nine VH gene families have been positioned with respect to the J558 and 3660 VH families in A/J (Ighe) as follows: 3609-J558-(J606,VGAM3-8,S107)-3660-(X24,Q52,7183 )-DH. Maps generated with respect to J558 in the BALB/c (Igha) and C57BL/6 (Ighb) strains are consistent with these results. The organization of the VH complex produced by deletion mapping is quite different from the accepted map generated by other methods, particularly in that J558 is more DH distal and 3660 is more DH proximal than previously thought. The order presented here is compatible with VH rearrangement frequencies suggesting preferential utilization of DH-proximal VH gene segments. Our data also indicate that interspersion of some VH family members may be a common feature of the murine VH complex since the 3609 VH family is interdigitated in the three strains and a Q52 VH gene segment is interspersed in C57BL/6.
缺失图谱分析已被用于对三种近交系小鼠品系中的重链可变区（VH）基因家族进行排序。这九个VH基因家族相对于A/J（Ighe）品系中的J558和3660 VH家族的定位如下：3609-J558-（J606、VGAM3-8、S107）-3660-（X24、Q52、7183）-DH。在BALB/c（Igha）和C57BL/6（Ighb）品系中相对于J558生成的图谱与这些结果一致。通过缺失图谱产生的VH复合体的组织与通过其他方法产生的公认图谱有很大不同，特别是J558比以前认为的更靠近DH远端，而3660比以前认为的更靠近DH近端。这里给出的顺序与VH重排频率一致，表明优先利用靠近DH的VH基因片段。我们的数据还表明，一些VH家族成员的散布可能是小鼠VH复合体的一个共同特征，因为3609 VH家族在这三个品系中相互交错，并且一个Q52 VH基因片段在C57BL/6中散布。
